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| Status |
Public on Feb 21, 2008 |
| Title |
Human in vivo nutrigenomic effect of olive oil |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Olive oil is protective against risk factors for cardiovascular and cancer diseases. A nutrigenomic approach was performed to assess whether olive oil, the main fat of the Mediterranean diet modifies the gene expression in human peripheral blood mononuclear cells. Six healthy male volunteers ingested, at fasting state, 50 ml of olive oil, and continued with the same olive oil as a source of raw fat (25ml/day) during 3 weeks. Prior to intervention a 1-week washout period with sunflower oil as the only source of fat was followed. During the 3 days before, and on the intervention day, a very low phenolic compound diet was followed. At baseline (0h), at post ingestion (6h), and at fasting state after 3 weeks of sustained consumption of olive oil total RNA was isolated from PBMC. Gene expression was evaluated by microarray and verified by qRT-PCR.
Keywords: Olive oil, gene expression, single dose, sustained consumption
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| Overall design |
Three pools of total RNA were prepared in triplicates (9 samples in total). Each pool refers to different time point of the study (0h-wash out, 6h postprandial and 3 weeks intervention). Pool at 0h serves as reference sample.
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| Contributor(s) |
Konstantinidou V, Khymenets O, Fitó M, de la Torre R, Anglada R, de Alarcón P, Dopazo A, Farré-Albaladejo M, Covas M |
| Citation(s) |
19545487, 19290808 |
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| Submission date |
Feb 20, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
MONTSERRAT FITO COLOMER |
| E-mail(s) |
mfito@imim.es
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| Organization name |
IMIM
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| Street address |
DR.AIGUADER 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL6511 |
ABI Human Genome Survey Microarray Version 1 |
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| Samples (9)
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| GSM266996 |
PMNCs_total RNA Pool_Wash Out_Aliquot N1 |
| GSM266997 |
PMNCs_total RNA Pool_Wash Out_Aliquot N2 |
| GSM266998 |
PMNCs_total RNA Pool_Wash Out_Aliquot N3 |
| GSM266999 |
PMNCs_total RNA Pool_PostPrandial_Aliquot N1 |
| GSM267000 |
PMNCs_total RNA Pool_PostPrandial_Aliquot N2 |
| GSM267001 |
PMNCs_total RNA Pool_PostPrandial_Aliquot N3 |
| GSM267002 |
PMNCs_total RNA Pool_3 weeks Intervention_Aliquot N1 |
| GSM267003 |
PMNCs_total RNA Pool_3 weeks Intervention_Aliquot N2 |
| GSM267004 |
PMNCs_total RNA Pool_3 weeks Intervention_Aliquot N3 |
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| Relations |
| BioProject |
PRJNA107743 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10590_RAW.tar |
11.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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