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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 22, 2017 |
| Title |
STAT4 and T-bet control follicular helper T cells development in viral infections [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Follicular helper T (Tfh) cells promote germinal center (GC) B cell survival and proliferation, and guide their differentiation and Ig isotype switching by delivering contact-dependent and soluble factors, including IL-21, IL-4, IL-9, and IFN-g. IL-21 and IFN-g are co-expressed by Tfh cells during acute and chronic infections, but transcriptional regulation of these cytokines in the GC is incompletely understood. We show that the Th1 transcriptional regulators T-bet and STAT4 are co-expressed with Bcl6 in Tfh cells following acute murine lymphocytic choriomeningitis virus infection, albeit with temporal decline in T-bet expression as the GC response evolved. T-bet was important for Tfh cell production of IFN-g, but not IL-21, and for the generation of a robust germinal center reaction. STAT4, phosphorylated in Tfh cells upon infection, was required for their expression of T-bet and Bcl6, and that of IFN-g and IL-21. These data indicate that T-bet is concomitantly expressed with Bcl6 in Tfh cells and is required alongside STAT4 phosphorylation to coordinate Tfh cell IL-21 and IFN-g production, and for promotion of the GC response following acute viral challenge.
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| Overall design |
Each group consisted of 5 mice pooled together. Two replicates of each group were used for ATAC-seq. Two groups were studied: Wild-type and homozygous STAT4 knockout mice.
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| Contributor(s) |
Weinstein JS, Laidlaw BJ, Lu Y, Wang JK, Schulz VP, Herman EI, Li N, Gallagher PG, Craft JE |
| Citation(s) |
29212666 |
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| Submission date |
Oct 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Vince Schulz |
| E-mail(s) |
vincent.schulz@yale.edu
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| Organization name |
Yale University
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| Department |
Department of Pediatrics
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| Lab |
Gallagher
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| Street address |
333 Cedar St. LMP 4085
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE105808 |
STAT4 and T-bet control follicular helper T cells development in viral infections |
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| Relations |
| BioProject |
PRJNA415459 |
| SRA |
SRP120995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE105807_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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