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| Status |
Public on Nov 21, 2018 |
| Title |
T47D RNA-seq and ChrRNA-seq data |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed genome-wide RNA-seq and ChrRNA-seq experiments in T47D cell lines after PADI2 knock down . Both the mRNA and chromatin RNA seq peformed in sicontrol and siPADI2 as in biological replicates . Also we performed mRNA seq in T47D cells expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2.
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| Overall design |
We designed experiment to perform mRNA sequencing in T47D after PADI2 knock down as in biological replicate experiemnt . Given the fact we found significant number of genes differentailly expressed after PADI2 knockdown in T47D cells, we designed to perform Chromatin RNA seq in T47D cells after PADI2 knock down as in biological replicates. We find that PADI2 deiminates R1810 at CTD (C-terminal domian) of RNAP2 , So in order to analyse the mRNA gene expression level in T47D cells expressing amanitin resistant HA - tagged wild type and mutant form of RNAP2 , we performed mRNA seq as in biological replicate in T47D cells expressing amanitin resistant HA - tagged wild type and mutant form of RNAP2.
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| Contributor(s) |
Sharma P, Lioutas A, Quilez J, Wright RH, Di Vona C, Le Dily F, Schüller R, Eick D, Beato M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
François Le Dily |
| E-mail(s) |
francois.ledily@crg.eu
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| Organization name |
CRG-Centre for Genomic Regulation
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| Street address |
Dr. Aiguader, 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE105795 |
Arginine citrullination at the C-terminal domain controls RNA Polymerase II transcription |
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| Relations |
| BioProject |
PRJNA415425 |
| SRA |
SRP120979 |
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