|
| Status |
Public on Nov 06, 2018 |
| Title |
Gene expression profiling of myofibroblasts in young and aged day 5 wound beds |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mesenchymal cells were isolated from day 5 dorsal skin wound beds of 7-weeks old and >24-months old using FACS. This experiment describes multiple unique subsets of wound bed myofibrolbasts capable of contributing to tissue repair.
|
| |
|
| Overall design |
Myofibroblast subsets were isolated by FACS. Cells were sorted based on CD34 and CD29 expression, where APs are CD34+ and CD29+; CD29 cells have high levels of CD29; and DN is CD34- and CD29 Low.
|
| |
|
| Contributor(s) |
Horsley V, Shook BA |
| Citation(s) |
30467144 |
| |
| Submission date |
Oct 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Brett Adam Shook |
| E-mail(s) |
brettshook@gwu.edu
|
| Organization name |
George Washington University
|
| Department |
Biochemistry & Molecular Medicine
|
| Street address |
2300 I St NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA415415 |
| SRA |
SRP120975 |
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