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| Status |
Public on Oct 23, 2017 |
| Title |
MBD2 Ablation Impairs Lymphopoiesis and Impedes Progression and Maintenance of T-cell Acute Lymphoblastic Leukemia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about the roles of methyl-CpG-binding domain protein 2 (MBD2), a “reader” of DNA methylation, in T-cell acute lymphoblastic leukemia (T-ALL). Here, we investigated the role of MBD2 in T-ALL by using an Mbd2 knockout mouse model. We found that MBD2 ablation impeded the progression and maintenance of Notch1-driven T-ALL.Our data reveals essential roles for MBD2 in lymphopoiesis and T-ALL and support an intriguing potential of MBD2 as a therapeutic target for T-ALL.
To explore potential mechanisms underlying the anti-ALL effects of MBD2 ablation, leukemic cells were harvested from mice transplanted with WT or Mbd2-/- T-ALL cells, and their global gene expression profiles (GEP) were compared. Double positive (DP) thymocytes were isolated and used as a normal control.
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| Overall design |
To understand the critical molecular events caused by MBD2 ablation in Notch1-driven T-ALL,the GFP+ leukemic mice BM cells were sorted from the recipients of MBD2-/- (TALL_M1,TALL_M2,TALL_M3) or MBD2+/+ (TALL_C1,TALL_C2,TALL_C3), and subjected to microarray analysis on Affymetrix microarrays.Furthermore, the normal DP (double positive) cells from steady-state mice thymus were chosen for examination as the other control groups.The DP cells were sorted from thymus of MBD2-/- (DP_M1,DP_M2,DP_M3) or MBD2+/+ (DP_C1,DP_C2,DP_C3) mice, and subjected to microarray analysis on Affymetrix microarrays.
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| Contributor(s) |
Zhou M, Zhou J |
| Citation(s) |
29330145 |
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| Submission date |
Oct 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Mi Zhou |
| E-mail(s) |
luckyday0920@163.com
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| Organization name |
Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology
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| Street address |
Jiefang Ave
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| City |
Wuhan |
| ZIP/Postal code |
430030 |
| Country |
China |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA415295 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE105763_RAW.tar |
40.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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