|
| Status |
Public on Aug 15, 2018 |
| Title |
Uridilation by TUT4/7 restricts retrotransposition of human Line-1s |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: the goal of this study was to test whether the amounts of genome-encoded Line-1s are influenced by TUTases and Mov10
Methods: RNA-Seq data were obtained for PA-1 or Hek293 Flp-IN T-Rex cells in which wild-type or mutant TUTases or Mov10 were overexpressed or the proteins were depleted by RNA interference
Results: Minor changes (less than 0.4-fold) were observed in the amounts of mRNAs of Homo sapiens-specific Line-1 families in Hek293 Flp-IN T-Rex and PA-1 either overexpressing or depleted of TUTases and Mov10
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| |
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| Overall design |
LINE-1 repetitive elements profiles of Hek293 Flp-IN T-Rex and PA-1 generated by deep sequencing, in triplicate, using Illumina NextSeq 500 and Illumina HiSeq 2500.
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| Contributor(s) |
Warkocki Z, Krawczyk PS, Adamska D, Bijata K, Garcia-Perez JL, Dziembowski A |
| Citation(s) |
30122351 |
| |
| Submission date |
Oct 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrzej Dziembowski |
| E-mail(s) |
adziembowski@iimcb.gov.pl
|
| Organization name |
International Institute Of Molecular And Cell Biology In Warsaw
|
| Lab |
Laboratory of RNA Biology
|
| Street address |
4 Ks. Trojdena Street
|
| City |
Warsaw |
| ZIP/Postal code |
02-109 |
| Country |
Poland |
| |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (49)
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| Relations |
| BioProject |
PRJNA415171 |
| SRA |
SRP120600 |
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