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Series GSE105173 Query DataSets for GSE105173
Status Public on Jul 01, 2019
Title DHX36 is the missing link to understand RNA G-quadruplex structures, mRNA stability, and translation [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary DHX36 is a ATP-dependent, 3´-5´ RNA helicase of the DEAH family. Previous publications reported this helicase to associate with AU-rich elements and to specifically unwind G-quadruplex structures. Here, we performed RNA-seq in triplicats of HEK293 wildtype cells and DHX36-KO-HEK293 cells to identify changes in RNA abundance upon presence or absence of this helicase. After sequencing and mapping to the human genome at nucleotide-resolution level we analysed the mRNA abundance of target mRNAs, identified in this study (see PAR-CLIP). Our analyses show that binding of DHX36 to 3´UTRS of target mRNAs significantly reduce their abundance.
Overall design Examination of RNA abundance in HEK293 wildtype cells and DHX36-KO-HEK293 cells
Contributor(s) Sauer M, Juranek SA, Benhalevy D, Wang X, Hafner M, Paeschke K
Citation(s) 31160600
Submission date Oct 19, 2017
Last update date Sep 30, 2019
Contact name Markus Sauer
Organization name University of Würzburg
Street address Am Hubland, Biozentrum - Lehrstuhl für Biochemie
City Würzburg
ZIP/Postal code 97074
Country Germany
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM2824220 Wildtype RNA-seq 1
GSM2824221 Wildtype RNA-seq 2
GSM2824222 Wildtype RNA-seq 3
This SubSeries is part of SuperSeries:
GSE105175 DHX36 resolves G-rich structures in mRNA untranslated region to allow their translation
BioProject PRJNA415034
SRA SRP120505

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Supplementary file Size Download File type/resource
GSE105173_RNAseq_gene_exp.diff.gz 821.7 Kb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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