NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE105158 Query DataSets for GSE105158
Status Public on Oct 30, 2017
Title RNA-Seq data on KMO inhibitor (CHDI-00340246) subchronic treated R6/2 mice.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Dysregulation of the kynurenine (Kyn) pathway has been associated with the progression of Huntington's disease (HD). In particular, elevated levels of the kynurenine metabolites 3-hydroxy kynurenine (3-OH-Kyn) and quinolinic acid (Quin), have been reported in the brains of HD patients as well as in rodent models of HD. The production of these metabolites is controlled by the activity of kynurenine mono-oxygenase (KMO), a mitochondrial outer membrane enzyme which catalyzes the synthesis of 3-OH-Kyn from Kyn. Thus inhibiting KMO is expected to produce a beneficial effect in Huntington's Disease (HD) patients, hopefully reversing their phenotype to match healthy subjects. To test this effect, we chronically treated a mouse model of HD (R6/2, a transgenic mouse model of HD which contains a human HTT gene containing 90 CAG repeats) and wild type mice with a KMO inhibitor for 8 weeks, and separately used a mock treatment on both the transgenic mice and wild type mice. The goal of this project is to analyze the RNA-seq data and find gene expression changes associated with the KMO inhibitor.
 
Overall design 12 wk old R6/2 and WT mice were treated per os twice-daily with 30 mg/kg CHDI-00340246 or vehicle (10% (w/v) HP-β-CD in 50mM Citrate Buffer (pH 5.5)) for 8 weeks (4-12 weeks of age). Cerebral cortex and striatum samples were taken from 23 mice, giving a total of 46 samples (half cortex and half striatum). RNA-seq data was collected on the three sets of mice. There were 8 HD+drug mice, 7 HD+mock mice, and 8 WT+mock mice. The mice were a mix of male and female.
 
Contributor(s) Aaronson J, Rosinski J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 19, 2017
Last update date May 15, 2019
Contact name Jeff Aaronson
E-mail(s) jeff.aaronson@chdifoundation.org
Phone (609) 945-9616
Organization name CHDI
Department Discovery & Systems Biology
Street address 155 Village Boulevard, Suite 200
City Princeton
State/province New Jersey
ZIP/Postal code 08540
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (46)
GSM2823447 R6/2_HD_Striatum_Female_CHDI-00340246_30mg/kg_replicate1
GSM2823448 R6/2_HD_Striatum_Female_CHDI-00340246_30mg/kg_replicate2
GSM2823449 R6/2_HD_Striatum_Male_Vehicle_replicate1
Relations
BioProject PRJNA414976
SRA SRP120493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE105158_KMO246_Subchronic_RNASeq_Counts_A-9444.xlsx.gz 9.9 Mb (ftp)(http) XLSX
GSE105158_KMO246_Subchronic_RNASeq_FPKM_A-9444.xlsx.gz 12.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap