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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 24, 2017 |
| Title |
The somatically generated T cell receptor CDR3a contributes to the MHC allele specificity of the T cell receptor |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Mature T cells bearing ab T cell receptors react with foreign antigens bound to alleles of major histocompatibility complex proteins (MHC) that they were exposed to during their development in the thymus, a phenomenon known as positive selection. The structural basis for positive selection has long been debated. Here, using mice expressing one of two different T cell receptor b chains and various MHC alleles, we show that positive selection-induced MHC bias of T cell receptors is affected both by the germline encoded elements of the T cell receptor a and b chain and, surprisingly, dramatically affected by the non germ line encoded CDR3 of the T cell receptor a chain. Thus, in addition to determining specificity for antigen, the non germline encoded elements of T cell receptors may help the proteins cope with the extremely polymorphic nature of major histocompatibility complex products within the species.
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| Overall design |
Naïve CD4 T cells were isolated from mice expressing a single T cell receptor b chain and one of 3 different alleles of MHCII. The T cell receptor a chains on these cells were sequenced and their sequences compared. Comparisons were also made in the use of different elements, the TRAVs, TRAJs and non germ line encoded CDR3 of the T cell receptor alpha chains.
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| Contributor(s) |
Marrack P, Krovi SH, Silberman D, White J, Kushnir E, Nakayama M, Crook J, Danhorn T, Leach SM, Anselment R, Scott-Browne J, Gapin L, Kappler JW |
| Citation(s) |
29148973 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI018785 |
Characteristics of T Cell Receptors |
NATIONAL JEWISH HEALTH |
Philippa C. Marrack |
| R01 DK099317 |
Molecular Dissection of Insulin Targeting in Anti-Islet Autoimmunity |
UNIVERSITY OF COLORADO DENVER |
Maki Nakayama |
| R01 AI092108 |
iNKT Cell Recognition of Endogenous Lipid Antigens |
UNIVERSITY OF COLORADO DENVER |
Laurent Gapin |
| T32 AI007405 |
Training Program in Immunology |
UNIVERSITY OF COLORADO DENVER |
Raul M Torres |
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| Submission date |
Oct 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Philippa Marrack |
| E-mail(s) |
marrackp@njhealth.org
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| Phone |
303 398 1324
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| Organization name |
National Jewish Health
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| Department |
Biomedical Research
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| Lab |
Marrack
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| Street address |
1400 Jackson St
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| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80206 |
| Country |
USA |
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| Platforms (3) |
| GPL16331 |
Ion Torrent PGM (Mus musculus) |
| GPL16815 |
454 GS FLX Titanium (Mus musculus) |
| GPL21744 |
454 GS Junior (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA414890 |
| SRA |
SRP120370 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE105129_RAW.tar |
152.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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