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| Status |
Public on Oct 14, 2017 |
| Title |
Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human embryonic stem cells (hESC) can be differentiated towards progeny resembling trophoblast upon exposure to BMP4. Here we analysed transcriptional changes in cells undergoing commitment to trophoblast at 6 time points using total RNA sequencing and identified early and late regulators of trophoblast fate.
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| Overall design |
hESC cell line H9 was differentiated towards trophoblast lineage using treatment with BMP4. Cells were collected at time points 0 hours (undifferentiated cells), 8 hours, 16 hours, 24 hours, 48 hours and 72 hours and subjected to total RNA sequencing using Illumina platform.
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| |
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| Contributor(s) |
Shaposhnikov D |
| Citation(s) |
29078328 |
| |
| Submission date |
Oct 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Dmitry Shaposhnikov |
| E-mail(s) |
dmitry.shaposhnikov@helmholtz-muenchen.de
|
| Organization name |
Helmholtz Center Munich
|
| Street address |
Ingolstädter Landstraße 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
|
| GSM2811246 |
undifferentiated hESC line H9 cells, biological replicate 1 |
| GSM2811247 |
undifferentiated hESC line H9 cells, biological replicate 2 |
| GSM2811248 |
hESC line H9 cells after 8 hours of BMP4 treatment, biological replicate 1 |
| GSM2811249 |
hESC line H9 cells after 8 hours of BMP4 treatment, biological replicate 2 |
| GSM2811250 |
hESC line H9 cells after 16 hours of BMP4 treatment, biological replicate 1 |
| GSM2811251 |
hESC line H9 cells after 16 hours of BMP4 treatment, biological replicate 2 |
| GSM2811252 |
hESC line H9 cells after 24 hours of BMP4 treatment, biological replicate 1 |
| GSM2811253 |
hESC line H9 cells after 24 hours of BMP4 treatment, biological replicate 2 |
| GSM2811254 |
hESC line H9 cells after 48 hours of BMP4 treatment, biological replicate 1 |
| GSM2811255 |
hESC line H9 cells after 48 hours of BMP4 treatment, biological replicate 2 |
| GSM2811256 |
hESC line H9 cells after 72 hours of BMP4 treatment, biological replicate 1 |
| GSM2811257 |
hESC line H9 cells after 72 hours of BMP4 treatment, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA414247 |
| SRA |
SRP119933 |
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