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Status |
Public on Jan 01, 2018 |
Title |
Ythdf2-mediated m6A mRNA clearance modulates neural development in mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
We found that the proliferation and differentiation capabilities of NSPCs decrease significantly in Ythdf2 null mutants.To explore the underlying molecular mechanism, we performed transcriptomics and well-established m6A-methylome analyses of NSPCs dervied from wild type and Ythdf2-/- embryo brains. RNA-seq data revealed that expressions of genes enriched in neural development pathways were significantly disturbed. The inhibitory genes, like Flrt2, Ptprd, et al. in regulation of JAK-STAT cascade, which contributes to the neuroprotection and neurite outgrowth, showed increased gene expressions and m6A enrichment by m6A-seq. We identified that without the recognizing and binding of Ythdf2, the degradation of neuron differentiation related m6A-modified mRNAs were delayed in Ythdf2-/-, thereby disturbing the proliferation and differentiation of NSPCs. In summary, our findings uncovered that Ythdf2 modulates neural developmental via regulating the clearance of mRNA targets.
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Overall design |
Examination of gene expression levels and m6A levels in mRNAs in wild type and Ythdf2 deficient mouse E14.5 derived NSPCs
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Contributor(s) |
Zhao X, Pan Q, Shi H, Lu Z |
Citation(s) |
29855337 |
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Submission date |
Oct 12, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Qingfei Pan |
E-mail(s) |
Qingfei.Pan@stjude.org
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Phone |
1-901-356-6214
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Organization name |
St. Jude Children's Research Hospital
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Department |
Department of Computational Biology
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Lab |
Yu Lab
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Street address |
262 Danny Thomas Place
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City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA414058 |
SRA |
SRP119834 |