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| Status |
Public on Dec 01, 2019 |
| Title |
Bone marrow-derived progenitors become decidual cells and are essential for implantation and pregnancy maintenance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The pregnant decidua is infiltrated by many immune cells which are thought to originate in the bone marrow (BM) promoting pregnancy. In addition, BM-derived progenitor cells (BMDPCs) become non-hematopoietic endometrial cells. However, whether BMDPCs become non-immune decidual cells and their functional contribution to pregnancy were previously unknown. Here, we show that embryo implantation stimulates vast BMDPCs recruitment to decidua, where BMDPCs differentiate into non-hematopoietic stromal decidual cells. In addition, to determine the functional importance of BMDPCs to pregnancy, we used mice with endometrial stromal-specific defects precluding successful pregnancy. BM transplant (BMT) from wild-type (WT) into Hoxa11-/- mice, which lack decidualization, results in uterine transcriptional changes leading to stromal expansion, gland formation, and marked decidualization otherwise absent in Hoxa11-/- mice. By contrast, BMT from Hoxa11-/- into WT mice induces pregnancy loss. Importantly, in Hoxa11+/- mice, which have increased pregnancy losses, BMT from WT donors leads to normalized expression of numerous decidualization-related genes and rescue of pregnancy loss. Collectively, these findings reveal that BMDPCs have a novel non-hematopoietic physiologic contribution to decidual stroma, thereby playing indispensable roles in pregnancy establishment and maintenance.
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| Overall design |
Female Hoxa11 heterozygotes or wild type mice were given Hoxa11 knockout or wild type bone marrow transplants. After mating, uterine implantation sites were collected and total RNA was extracted on ED 5.5. 14 samples were sequenced by single-end next generation sequencing and differential transcriptome analysis.
Single cell RNA analysis of the E9.5 implantation site in mice using the same GFP BMT model to further establish the decidual stromal phenotype of GFP+ BMDC cell subsets.
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| Contributor(s) |
Tal R, Shaikh S, Pallavi P, Tal A, Lopez-Giraldez F, Lyu F, Fang Y, Chinchanikar S, Liu Y, Kliman HJ, Alderman III M, Pluchino N, Kayani J, Mamillapalli R, Krause DS, Taylor HS |
| Citation(s) |
31513564 |
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| Submission date |
Oct 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Francesc Lopez |
| E-mail(s) |
francesc.lopez@yale.edu
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| Organization name |
Yale University
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| Department |
Department of Genetics
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| Lab |
YCGA
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| Street address |
P.O. Box 27386
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| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA413977 |
| SRA |
SRP119784 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104842_RAW.tar |
75.1 Mb |
(http)(custom) |
TAR (of MTX, TSV, XLSX) |
| GSE104842_gene_exp.diff.gz |
2.5 Mb |
(ftp)(http) |
DIFF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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