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| Status |
Public on Jan 23, 2019 |
| Title |
DNA hypermethylation encroachment at CpG island borders in cancer is predisposed by H3K4 monomethylation [WGBS_Mm] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The crosstalk between H3K4 monomethylation and DNA methylation has been receiving little attention to date. We investigated a mouse model harboring a loss-of-function mutation of lysine methyltransferase 2D (KMT2D), which catalyzes the monomethylation of lysine 4 on histone H3. We performed H3K4me1 ChIP-seq in spleen B cells with the wild type (WT) KMT2D as well as heterozygous (HET) and homozygous (HOM) chr15: 98,835,228 A>T KMT2D mutation. Alongside, using WGBS, we performed genome-wide DNA methylation profiling in spleen B cells with the WT KMT2D as well as HET and HOM chr15: 98,835,228 A>T KMT2D mutation.
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| Overall design |
Genome-wide analysis of DNA methylation using WGBS in B cells and blood from mice with the wild type KMT2D, heterozygous Ile5482Asn KMT2D mutation and homozygous Ile5482Asn KMT2D mutation.
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| Contributor(s) |
Skvortsova K, Masle-Farquhar E, Luu P, Clark SJ, Stirzaker C |
| Citation(s) |
30753827 |
| |
| Submission date |
Oct 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ksenia Skvortsova |
| E-mail(s) |
k.skvortsova@garvan.org.au
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| Phone |
478135210
|
| Organization name |
Garvan Institute
|
| Department |
Genomics and Epigenetics
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| Lab |
Developmental Epigenomics
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| Street address |
384 Victoria St, Darlinghurst
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE104791 |
DNA hypermethylation encroachment at CpG island borders in cancer is predisposed by H3K4 mono-methylation patterns. |
|
| Relations |
| BioProject |
PRJNA413838 |
| SRA |
SRP119705 |
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