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Series GSE104727 Query DataSets for GSE104727
Status Public on Sep 24, 2019
Title Distinct populations of embryonic epithelial progenitors generate Lgr5+ intestinal stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To follow the changes in the transcriptional programs accompanying the specification of the embryonic Lgr5+ cells we sequenced whole transcriptomes of embryonic intestinal epithelium progenitors (at E13.5 and E15.5). EpCAM positive embryonic gut epithelium was isolated from dissected small intestines using fluorescence activated cell sorting (FACS). Lgr5+ progenitors were purified on the basis of GFP fluorescence from Lgr5GFP-Cre-ERT mice (Barker et al. 2007). Double positive embryonic progenitors were isolated by FACS based on GFP and tdTomato fluorescence.
 
Overall design Intestinal epithelial cells from two embryonic stages (E13.5 and E15.5) were used. For embryonic stages biological triplicates were analysed.
 
Contributor(s) Soshnikova N
Citation(s) 29037931
Submission date Oct 09, 2017
Last update date Jul 25, 2021
Contact name Sergi Sayols
Organization name Institute of Molecular Biology, Mainz
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (45)
GSM2806704 Axin2:tdTomato+_E13.5_intestinal epitheliua cells labelled at E9.5_wt_rep1
GSM2806705 Axin2:tdTomato+_E13.5_intestinal epitheliua cells labelled at E9.5_wt_rep2
GSM2806706 Axin2:tdTomato+_E13.5_intestinal epitheliua cells labelled at E9.5_wt_rep3
Relations
BioProject PRJNA413664
SRA SRP119596

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Supplementary file Size Download File type/resource
GSE104727_RAW.tar 4.2 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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