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| Status |
Public on Jan 08, 2018 |
| Title |
S1P-dependent inter-organ trafficking of group 2 innate lymphoid cells supports host defense |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Innate lymphoid cells (ILCs) are considered to be the innate counterparts of adaptive T lymphocytes and play important roles in host defense, tissue repair, metabolic homeostasis, and inflammatory diseases. ILCs are generally thought of as tissue-resident cells, but whether ILCs strictly behave in a tissue-resident manner or can move between sites during infection is unclear. We show here that IL-25- or helminthic infection-induced inflammatory ILC2s are not tissue-resident but circulating cells, which arise from resting ILC2s residing in intestinal lamina propria and then migrate to mesenteric lymph nodes, spleen, lung, and liver. IL-25 induces rapid proliferation of the intestinal ILC2s and a change in their sensitivity to S1P-mediated chemotaxis, leading to lymphatic entry, blood circulation, and accumulation in periphery sites, including the lung where they contribute to anti-helminth defense and tissue repair. Our finding of cytokine-driven expansion and migration of innate lymphocytes, a behavioral parallel to the antigen-driven priming, expansion, and migration of adaptive lymphocytes to effector sites in distant tissues, provides a significant advance in our overall understanding of ILCs and indicates that ILCs complement adaptive immunity by providing both local and distant site effector protection during infection.
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| Overall design |
We examined the transcriptomes of BM ILC2 progenitors, lung nILC2s, IL-33-activated lung nILC2s, intestinal ILC2s, IL-25-induced lung iILC2s, and MLN iILC2s by RNA-Seq.
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| Contributor(s) |
Huang Y, Mao K, Chen X, Sun M, Kawabe T, Li W, Usher N, Zhu J, Urban JF Jr, Paul WE, Germain RN |
| Citation(s) |
29302015 |
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| Submission date |
Oct 06, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Mingan Sun |
| E-mail(s) |
mingansun@gmail.com
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| Organization name |
National Institute of Health
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| Department |
Eunice Kennedy Shriver National Institute of Child Health and Human Development
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| Street address |
Building 6B, 6 Center Drive
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20814 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA413546 |
| SRA |
SRP119537 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104708_RAW.tar |
7.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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