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| Status |
Public on Feb 22, 2018 |
| Title |
Characterization of transcriptomics landscape in HUVEC cells exposed to oxidative stress (Total RNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The aim of the project was to characterize the transcriptional landscape of human HUVEC cells exposed to oxidative stress (oxstress). In order to do so cell cultures have been exposed to 200uM H2O2 for either 16 hours or 36 hours to induce oxstress. Total ribodepleted RNA obtained from both time points have been sequenced and small RNA for the 16 hours time point have been sequenced as well. Datasets have been characterized and overlapped. This entry contains the dataset of total ribodepleted RNA.
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| |
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| Overall design |
Three conditions are available: control untreated HUVEC cells, HUVEC cells exposed to 200uM H2O2 for 16 hours, HUVEC cells exposed to 200uM H2O2 for 36h. Each condition is available in triplicate, for a total of 9 samples.
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| Contributor(s) |
Martelli F, Matteo C, Garcia-Manteiga J, Fuschi P |
| Citation(s) |
29242407 |
| |
| Submission date |
Oct 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Fabio Martelli |
| E-mail(s) |
fabio.martelli@grupposandonato.it
|
| Phone |
+390252774533
|
| Organization name |
IRCCS-Policlinico San Donato
|
| Lab |
Molecular Cardiology
|
| Street address |
via morandi 30
|
| City |
Milan |
| ZIP/Postal code |
20097 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (9)
|
| GSM2805213 |
Oxstress total RNA h2o2 16h replicate 1 |
| GSM2805214 |
Oxstress total RNA h2o2 16h replicate 2 |
| GSM2805215 |
Oxstress total RNA h2o2 16h replicate 3 |
| GSM2805216 |
Oxstress total RNA h2o2 36h replicate 1 |
| GSM2805217 |
Oxstress total RNA h2o2 36h replicate 2 |
| GSM2805218 |
Oxstress total RNA h2o2 36h replicate 3 |
| GSM2805219 |
Oxstress total RNA control replicate 1 |
| GSM2805220 |
Oxstress total RNA control replicate 2 |
| GSM2805221 |
Oxstress total RNA control replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE104666 |
Characterization of transcriptomics landscape in HUVEC cells exposed to oxidative stress |
|
| Relations |
| BioProject |
PRJNA413424 |
| SRA |
SRP119487 |
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