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| Status |
Public on Mar 04, 2008 |
| Title |
Human Skin: Before and 1 week after Isotretinoin Treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The pathogenesis of acne has been linked to multiple factors such as increased sebum production, inflammation, follicular hyperkeratinization, and the action of Propionibacterium acnes within the follicle. 13-cis Retinoic Acid (13-cis RA, isotretinoin) is the most potent agent in acne treatment. Surprisingly, its mechanism of action in acne is still unknown. Gene expression profiling of skin from 6 patients treated with isotretinoin was performed to gain insights into its mechanism of action. Skin biopsies were obtained from the patients at baseline and at one-week isotretinoin treatment. Gene array expression profiling was conducted using Affymetrix HG-U133A 2.0 arrays in order to examine changes in gene expression as a result of treatment. After treatment, 43 genes were significantly changed: 38 up-regulated and 5 down-regulated. A significant proportion of these genes are involved in pathways that regulate differentiation, tumor suppression, serine proteases, serine protease inhibitors and solute transfer. These studies are the first describing the initial changes in gene expression associated with isotretinoin therapy and are valuable in identifying potential therapeutic targets in acne.
Keywords: drug treatment effects
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| Overall design |
Total 12 chips: 6 baseline/before isotretinoin and 6 after 1-week isotretinoin treatment.
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| |
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| Contributor(s) |
Thiboutot DM |
| Citation(s) |
18317594 |
|
Nelson AM, Zhao W, Gilliland KL, Zaenglein AL, Liu W, Thiboutot DM. Early gene changes induced by isotretinoin in the skin provide clues to its mechanism of action. Dermatoendocrinol. 2009 Mar-Apr;1(2):100-101.
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| |
| Submission date |
Feb 07, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Diane M Thiboutot |
| E-mail(s) |
dthiboutot@psu.edu
|
| Organization name |
The Pennsylvannia State University College of Medicine
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| Department |
Department of Dermatology; Jake Gittlen Res. Fnd.
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE10434 |
Retinoic acid effect on sebocytes and the skin |
|
| Relations |
| BioProject |
PRJNA108919 |
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