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Series GSE10433 Query DataSets for GSE10433
Status Public on Mar 04, 2008
Title Human Skin: Before and 1 week after Isotretinoin Treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The pathogenesis of acne has been linked to multiple factors such as increased sebum production, inflammation, follicular hyperkeratinization, and the action of Propionibacterium acnes within the follicle. 13-cis Retinoic Acid (13-cis RA, isotretinoin) is the most potent agent in acne treatment. Surprisingly, its mechanism of action in acne is still unknown. Gene expression profiling of skin from 6 patients treated with isotretinoin was performed to gain insights into its mechanism of action. Skin biopsies were obtained from the patients at baseline and at one-week isotretinoin treatment. Gene array expression profiling was conducted using Affymetrix HG-U133A 2.0 arrays in order to examine changes in gene expression as a result of treatment. After treatment, 43 genes were significantly changed: 38 up-regulated and 5 down-regulated. A significant proportion of these genes are involved in pathways that regulate differentiation, tumor suppression, serine proteases, serine protease inhibitors and solute transfer. These studies are the first describing the initial changes in gene expression associated with isotretinoin therapy and are valuable in identifying potential therapeutic targets in acne.
Keywords: drug treatment effects
Overall design Total 12 chips: 6 baseline/before isotretinoin and 6 after 1-week isotretinoin treatment.
Contributor(s) Thiboutot DM
Citation(s) 18317594
Nelson AM, Zhao W, Gilliland KL, Zaenglein AL, Liu W, Thiboutot DM. Early gene changes induced by isotretinoin in the skin provide clues to its mechanism of action. Dermatoendocrinol. 2009 Mar-Apr;1(2):100-101.
Submission date Feb 07, 2008
Last update date Dec 06, 2018
Contact name Diane M Thiboutot
Organization name The Pennsylvannia State University College of Medicine
Department Department of Dermatology; Jake Gittlen Res. Fnd.
Street address 500 University Drive
City Hershey
State/province PA
ZIP/Postal code 17033
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM263921 Patient 1 skin baseline
GSM263922 Patient 1 skin treated
GSM263923 Patient 2 skin baseline
This SubSeries is part of SuperSeries:
GSE10434 Retinoic acid effect on sebocytes and the skin
BioProject PRJNA108919

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10433_RAW.tar 39.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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