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| Status |
Public on Jun 28, 2018 |
| Title |
PP1-NIPP1 wild type and mutant overexpression in Flip-In™ T-Rex Hela cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNAi-seq of stable Flp-In™ T-Rex Hela cells that inducibly expressed PP1-NIPP1 and mutants.
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| Overall design |
Four cell types are investigated under two conditions in three independent experiments, resulting in 24 samples in total (i.e. 4x2x3). The four cell types are: (1) Flp-In™ T-Rex Hela cells with doxycycline-inducible EGFP transgene, (2) Flp-In™ T-Rex Hela cells with doxycycline-inducible EGFP-PP1-NIPP1 transgene, (3) Flp-In™ T-Rex Hela cells with doxycycline-inducible EGFP-PP1m-NIPP1 transgene (PP1 is mutated at Aa 64, i.e. D64A), (4) Flp-In™ T-Rex Hela cells with doxycycline-inducible EGFP-PP1-NIPP1m transgene (NIPP1 is mutated at the position AA 68-71, i.e. alanine mutation of NIPP1 residues 68-71). The two conditions are (1) not exposed to doxycycline, (2) treated with doxycycline. Three replicates are collected from three independent experiments.
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| |
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| Contributor(s) |
Winkler C, Van Eynde A |
| Citation(s) |
29898919 |
| |
| Submission date |
Sep 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
Nucleomics.Bioinformatics@vib.be
|
| Organization name |
VIB
|
| Department |
Nucleomics Core
|
| Street address |
Herestraat 49 Box 816
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA412362 |
| SRA |
SRP118980 |
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