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Series GSE104236 Query DataSets for GSE104236
Status Public on Sep 26, 2017
Title E. fischeriana root compound Dpo activates antiviral innate immunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary E. fischeriana has long been used as a traditional Chinese medicine.Recent studies reported that some compounds of E. fischeriana exhibited antimicrobial and immune enhance activity. Innate immune system is essential for the immune surveillance of inner and outer threats, initial host defense responses and immune modulation. The role of natural drug compounds, including E. fischeriana, in innate immune regulation is largely unknown. Here we demonstrate that E. fischeriana compound Dpo is involved in antiviral signaling. The genome wide RNA-seq analysis revealed that Dpo synergized the induction of ISGs by viral infection. Consistently, Dpo enhanced the antiviral immune responses and protected the mice from death during viral infection. Dpo however was not able to rescue STING deficient mice lethality caused by HSV-1 infection. The enhancement of ISG15 by Dpo was also impaired in STING, IRF3, IRF7 or ELF4 deficient cells, demonstrating that Dpo activates innate immune responses in a STING/IRFs/ELF4 dependent way. The STING/IRFs/ELF4 axis is therefore important for Dpo induced ISGs expression, and can be used by host to counteract infection.
Overall design immortallized bone marrow derived macrophages were treated with medium (control) or Dpo at a concentration of 8ng/ul for 12 hours (Dpo)before harvest for RNA extraction.
Contributor(s) You F, Chen J
Citation(s) 29124042
Submission date Sep 25, 2017
Last update date May 15, 2019
Contact name fuping you
Organization name Peking University Health Science Center
Street address xueyuan street
City beijing
ZIP/Postal code 100083
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM2793623 immortallized bone marrow derived macrophages treated with control medium
GSM2793624 immortallized bone marrow derived macrophages treated with Dpo at a concentration of 8ng/ul for 12 hours
BioProject PRJNA412099
SRA SRP118845

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GSE104236_con_VS_424_All_Gene.xls.gz 502.3 Kb (ftp)(http) XLS
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