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| Status |
Public on Sep 26, 2017 |
| Title |
E. fischeriana root compound Dpo activates antiviral innate immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
E. fischeriana has long been used as a traditional Chinese medicine.Recent studies reported that some compounds of E. fischeriana exhibited antimicrobial and immune enhance activity. Innate immune system is essential for the immune surveillance of inner and outer threats, initial host defense responses and immune modulation. The role of natural drug compounds, including E. fischeriana, in innate immune regulation is largely unknown. Here we demonstrate that E. fischeriana compound Dpo is involved in antiviral signaling. The genome wide RNA-seq analysis revealed that Dpo synergized the induction of ISGs by viral infection. Consistently, Dpo enhanced the antiviral immune responses and protected the mice from death during viral infection. Dpo however was not able to rescue STING deficient mice lethality caused by HSV-1 infection. The enhancement of ISG15 by Dpo was also impaired in STING, IRF3, IRF7 or ELF4 deficient cells, demonstrating that Dpo activates innate immune responses in a STING/IRFs/ELF4 dependent way. The STING/IRFs/ELF4 axis is therefore important for Dpo induced ISGs expression, and can be used by host to counteract infection.
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| Overall design |
immortallized bone marrow derived macrophages were treated with medium (control) or Dpo at a concentration of 8ng/ul for 12 hours (Dpo)before harvest for RNA extraction.
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| Contributor(s) |
You F, Chen J |
| Citation(s) |
29124042 |
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| Submission date |
Sep 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
fuping you |
| E-mail(s) |
fupingyou@hsc.pku.edu.cn
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| Organization name |
Peking University Health Science Center
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| Street address |
xueyuan street
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| City |
beijing |
| ZIP/Postal code |
100083 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
| GSM2793623 |
immortallized bone marrow derived macrophages treated with control medium |
| GSM2793624 |
immortallized bone marrow derived macrophages treated with Dpo at a concentration of 8ng/ul for 12 hours |
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| Relations |
| BioProject |
PRJNA412099 |
| SRA |
SRP118845 |
