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Series GSE104226 Query DataSets for GSE104226
Status Public on Sep 22, 2020
Title Translated Long Non-Coding Ribonucleic Acid ZFAS1 Promotes Cancer Cell Migration by Elevating Reactive Oxygen Species Production in Hepatocellular Carcinoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Micropeptides (≤100 amino acids) are essential regulators of physiological and pathological processes, which can be encoded by small open reading frames (smORFs) derived from long non-coding RNAs (lncRNAs). Recently, lncRNA-encoded micropeptides have been shown to have essential roles in tumorigenesis. Since translated smORF identification remains technically challenging, little is known of their pathological functions in cancer. Therefore, we created classifiers to identify translated smORFs derived from lncRNAs based on ribosome-protected fragment sequencing and machine learning methods. In total, 537 putative translated smORFs were identified and the coding potential of five smORFs was experimentally validated via green fluorescent protein-tagged protein generation and mass spectrometry. After analyzing 11 lncRNA expression profiles of seven cancer types, we identified one validated translated lncRNA, ZFAS1, which was significantly up-regulated in hepatocellular carcinoma (HCC). Functional studies revealed that ZFAS1 can promote cancer cell migration by elevating intracellular reactive oxygen species production by inhibiting nicotinamide adenine dinucleotide dehydrogenase expression, indicating that translated ZFAS1 may be an essential oncogene in the progression of HCC. In this study, we systematically identified translated smORFs derived from lncRNAs and explored their potential pathological functions in cancer to improve our comprehensive understanding of the building blocks of living systems
Overall design Transcriptomeanalyses of ZFAS1 overexpression and si in SK-Hep1 human hepatoma cell lines by deep sequencing. In total transcriptome data set, include two PCDH control, two ZFAS ORF overexpression, two si GFP, and two si ZFAS1
Web link
Contributor(s) Guo Z, Meng Y, Zhai X, Xie C, Zhao N, Li M, Zhou C, Li K, Liu T, Yang X, Wu Y
Citation(s) 31781169
Submission date Sep 25, 2017
Last update date Jul 25, 2021
Contact name zhiwei Guo
Organization name Southern medical university
Street address NO.1838 Guangzhou Road
City Guangzhou
State/province Guangdong
ZIP/Postal code 510515
Country China
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (8)
GSM2792919 PCDH_mRNA_rep1
GSM2792920 PCDH_mRNA_rep2
GSM2792921 ZFAS1_ORF_rep1
BioProject PRJNA412082
SRA SRP118825

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Supplementary file Size Download File type/resource
GSE104226_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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