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| Status |
Public on Jan 25, 2018 |
| Title |
Expression analysis of Fudulus heteroclitus populations exposed to arsenic during hypo-osmotic shock |
| Organism |
Fundulus heteroclitus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression was measured in the gills of saltwater (SW) acclimated Atlantic Killifish, Fundulus heteroclitus, over a time course of freshwater (FW) exposure, in which half the fish were exposed to arsenic.
Fish were sampled from three populations, two from Maine, USA (ME) and one from Virginia, USA (VA)
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| Overall design |
72 samples comparing gene expression in killifish fish gills from three populations during acclimation to fresh water: 24 from Northeast Creek, Maine (ME1), 24 from Horseshoe Creek, Maine (ME2), and 24 King’s Creek, Virginia (VA). Half of the freshwater acclimation time course experiments included exposure to 100 micrograms per liter arsenic, as follows. For each population, groups of 4 fish were exposed to: seawater (0 hours freshwater), seawater and arsenic (0 hours freshwater), freshwater for 1 hour, freshwater for 1 h with arsenic, freshwater for 24 hours, freshwater for 24 hours with arsenic.
Gene expression measurements were made with custom NimbleGen platform GPL14994, which has 135,000 probes that map to 34,713 gene models and 16,104 unique Fundulus heteroclitus genes based on a high-quality killifish reference genome (PMID:28201664)
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| Contributor(s) |
Shaw JR, Hampton TH, Glaholt SP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 25, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Thomas H. Hampton |
| E-mail(s) |
Thomas.H.Hampton@Dartmouth.edu
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| Organization name |
Geisel School of Medicine at Dartmouth
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| Department |
Microbiology and Immunology
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| Lab |
Bruce Stanton
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| Street address |
Dewey Field Road, HB 7550
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| City |
Hanover |
| State/province |
NH |
| ZIP/Postal code |
03755 |
| Country |
USA |
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| Platforms (1) |
| GPL14994 |
NimbleGen_Fundulus heteroclitus_Expression Array_12x135k_All Probes [DesignID 11308] |
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| Samples (72)
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| Relations |
| BioProject |
PRJNA412045 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104218_RAW.tar |
299.0 Mb |
(http)(custom) |
TAR (of PAIR, TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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