|
| Status |
Public on May 31, 2018 |
| Title |
Quantitative proteomic characterization and comparison of T helper 17 and iTreg cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using mass spectrometry-based label-free quantitative (LFQ) proteomics analysis of in vitro differentiated murine Th17 and induced T regulatory (iTreg) cells. More than 4000 proteins covering almost all subcellular compartments were detected. Quantitative comparison of the protein expression profiles resulted in the identification of proteins specifically expressed in the Th17 and iTreg cells. Importantly, our combined analysis of proteome and gene expression data revealed protein expression changes that were not associated with changes at the transcriptional level.
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| Overall design |
Naïve CD4+ T cells were isolated from 8–10-week-old C57BL/6 mice spleens. Cells were polarized to Th0, Th17 and iTreg conditions for three days. Four biological replicates were prepared for RNA-seq.
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| Contributor(s) |
Zhi C, Harri L, Imran M, Kari N, Inna S, Anne R, David G, Santosh D B, Robert M |
| Citation(s) |
29851958 |
| |
| Submission date |
Sep 22, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Imran Ahammad Mohammad |
| E-mail(s) |
imrmoh@utu.fi
|
| Organization name |
Turku center for Biotechnology
|
| Department |
Lymphocytes and Inflammation
|
| Lab |
Chen,Zhi
|
| Street address |
Tykistokatu 6
|
| City |
Turku |
| State/province |
Finland |
| ZIP/Postal code |
20540 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA411825 |
| SRA |
SRP118695 |
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