|
| Status |
Public on Sep 22, 2017 |
| Title |
Epidermal growth factor receptor expression licenses T helper-2 cells to function in a T cell receptor-independent fashion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gastro-intestinal helminth infections trigger the release of interleukin-33 (IL-33), which induces type 2 helper T-cells (Th2 cells) at the site of infection to produce IL-13, thereby contributing to host resistance in a T cell receptor (TCR)-independent manner. Here we show that as a prerequisite for IL-33 induced IL-13 secretion, Th2 cells required the expression of the Epidermal Growth Factor Receptor (EGFR) and of its ligand, Amphiregulin, for the formation of a signaling complex between T1/ST2 (the IL-33R) and EGFR. This shared signaling complex allowed IL-33 to induce the EGFR-mediated activation of the MAP-kinase signaling pathway and consequently the expression of IL-13. Lack of EGFR expression on T cells abrogated IL-13 expression in infected tissues and impaired host resistance. EGFR expression on Th2 cells was TCR-signaling dependent, and therefore our data reveal a mechanism by which antigen-presentation controls the innate effector function of Th2 cells at the site of inflammation.
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| |
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| Overall design |
RNA sequencing of CD4+ CD25− CD45Rbhi cells from spleens of wt and EGFRΔCD4 mice
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| |
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| Contributor(s) |
Mokry M |
| Citation(s) |
29045902 |
| |
| Submission date |
Sep 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michal Mokry |
| E-mail(s) |
m.mokry@umcutrecht.nl
|
| Organization name |
Wilhelmina Children's Hospital, University Medical Center Utrecht
|
| Street address |
Lundlaan 6
|
| City |
Utrecht |
| ZIP/Postal code |
3584 EA |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA408233 |
| SRA |
SRP118470 |
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