|
| Status |
Public on Oct 01, 2018 |
| Title |
Long-term in vitro expansion of epithelial stem cells enabled by pharmacological inhibition of PAK1-ROCK-Myosin II and TGF-β signaling (MeDIP-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Despite substantial self-renewal capability in vivo, epithelial stem and progenitor cells located in various tissues expand for a few passages in vitro in feeder-free condition before they succumb to growth arrest. Here we describe the EpiX™ method that utilizes small molecules that inhibit PAK1-ROCK-Myosin II and TGF-β signaling to achieve over one trillion-fold expansion of human epithelial stem and progenitor cells from skin, airway, mammary and prostate glands in the absence of feeder cells. Transcriptomic and epigenomic studies show that this condition helps epithelial cells to overcome stresses for continuous proliferation. EpiX-expanded basal epithelial cells differentiate into mature epithelial cells consistent with their tissue origins. Whole genome sequencing reveals that the cells retain remarkable genome integrity after extensive in vitro expansion without acquiring tumorigenicity. EpiX technology provides a solution to exploit the potential of tissue-resident epithelial stem and progenitor cells for regenerative medicine.
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| |
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| Overall design |
MeDIP profiles of human foreskin keratinocytes in different culture conditions were generated by deep sequencing, in duplicate, using Illumina NextSeq.
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| |
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| Contributor(s) |
Lee H, Zhang C, Wang T |
| Citation(s) |
30332641 |
| |
| Submission date |
Sep 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hyung Joo Lee |
| Organization name |
Washington University School of Medicine
|
| Department |
Department of Genetics
|
| Lab |
Ting Wang
|
| Street address |
4515 McKinley Avenue
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE103759 |
Long-term in vitro expansion of epithelial stem cells enabled by pharmacological inhibition of PAK1-ROCK-Myosin II and TGF-β signaling |
|
| Relations |
| BioProject |
PRJNA406937 |
| SRA |
SRP117341 |
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