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| Status |
Public on Aug 03, 2018 |
| Title |
A map of gene expression in neutrophil-like cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report gene expression data for the human cell lines HL-60 and PLB-985, which serve as models for human neutrophils. We measured gene expression using RNA-Seq for these cell lines both prior and after differentiation into a neutrophil-like state using two differentiation protocols (treatment with DMSO or treatment with DMSO and replacement of serum with Nutridoma).
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| Overall design |
HL-60 and PLB-985 cells grown in culture were processed for RNA-Seq both before and after differentiation for six days in media supplemented with 1.3% dimethyl sulfoxide (DMSO). The cell lines were also analyzed after differentiation for six days in media with 1.3% DMSO, reduced serum (0.5% FBS), and Nutridoma-CS (2%). PLB-985 cells were also analyzed at intermediate time points of 2 days and 4 days with the Nutridoma protocol.
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| Contributor(s) |
Rincón E, Rocha-Gregg BL, Collins SR |
| Citation(s) |
30068296 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| DP2 HD094656 |
Rewiring Control of Leukocyte Motility for Therapeutic Application |
UNIVERSITY OF CALIFORNIA DAVIS |
Sean Ryan Collins |
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| Submission date |
Sep 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Sean R. Collins |
| E-mail(s) |
srcollins@ucdavis.edu
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| Organization name |
UC Davis
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| Department |
Microbiology & Molecular Genetics
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| Street address |
One Shields Ave, 334 Briggs Hall
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| City |
Davis |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (14)
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| GSM2779509 |
PLB-985, DMSO+Nutridoma, day 6, replicate 1 |
| GSM2779510 |
HL-60, not differentiated, replicate 1 |
| GSM2779511 |
HL-60, DMSO, day 6, replicate 1 |
| GSM2779512 |
HL-60, DMSO+Nutridoma, day 6, replicate 1 |
| GSM2779513 |
PLB-985, not differentiated, replicate 2 |
| GSM2779514 |
PLB-985, DMSO, day 6, replicate 2 |
| GSM2779515 |
PLB-985, DMSO+Nutridoma, day 2 |
| GSM2779516 |
PLB-985, DMSO+Nutridoma, day 6, replicate 2 |
| GSM2779517 |
HL-60, not differentiated, replicate 2 |
| GSM2779518 |
HL-60, DMSO, day 6, replicate 2 |
| GSM2779519 |
HL-60, DMSO+Nutridoma, day 6, replicate 2 |
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| Relations |
| BioProject |
PRJNA404567 |
| SRA |
SRP117267 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103706_Merged_data_FPKM_and_normalized.xlsx |
15.3 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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