|
| Status |
Public on Jan 31, 2018 |
| Title |
Transcriptome-wide response to synthetic chromatin protein PcTF: Breast Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to identify genes that become upregulated in response to a synthetic transcription factor, PcTF, in a panel of breast cancer model cell lines. PcTF contains a conserved H3K27me3-binding chromodomain expressed in-frame with an mCherry fluorescent tag and a VP64 activation domain. Therefore, we expected genes with H3K27me3-associated promoters to become upregulated upon PcTF expression. We observed that hundreds of genes became up or downregulated.
|
| |
|
| Overall design |
MCF7, BT474, or BT549 cells were transfected with a plasmid that constitutively expressed the PcTF protein. 24, 48, and 72 hrs post-transfection, total mRNA was extracted from treated and untreated cells, as well as from untransfected MCF10A cells.
|
| |
|
| Contributor(s) |
Haynes KA, Wilson Sayres MA, Olney KC, Nyer DB |
| Citation(s) |
30253781 |
| |
| Submission date |
Sep 06, 2017 |
| Last update date |
Jan 07, 2020 |
| Contact name |
Karmella Haynes |
| E-mail(s) |
Karmella.Haynes@asu.edu
|
| Phone |
480-965-4636
|
| Organization name |
Arizona State University
|
| Department |
Biological and Health Systems Engineering
|
| Lab |
Haynes
|
| Street address |
427 E Tyler Mall
|
| City |
Tempe |
| State/province |
Arizona |
| ZIP/Postal code |
85281 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA401827 |
| SRA |
SRP117021 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103520_ALL_BT474_48rep1_rm_gene_exp.diff.gz |
145.0 Mb |
(ftp)(http) |
DIFF |
| GSE103520_ALL_gene_exp.diff.gz |
144.9 Mb |
(ftp)(http) |
DIFF |
| GSE103520_BT474_48rep1_rm_gene_exp.diff.gz |
10.6 Mb |
(ftp)(http) |
DIFF |
| GSE103520_BT474_gene_exp.diff.gz |
10.6 Mb |
(ftp)(http) |
DIFF |
| GSE103520_BT549_gene_exp.diff.gz |
11.3 Mb |
(ftp)(http) |
DIFF |
| GSE103520_MCF10A_BT474_48rep1_rm_gene_exp.diff.gz |
18.3 Mb |
(ftp)(http) |
DIFF |
| GSE103520_MCF10A_BT474_gene_exp.diff.gz |
18.3 Mb |
(ftp)(http) |
DIFF |
| GSE103520_MCF10A_BT549_gene_exp.diff.gz |
19.4 Mb |
(ftp)(http) |
DIFF |
| GSE103520_MCF10A_MCF7_gene_exp.diff.gz |
18.9 Mb |
(ftp)(http) |
DIFF |
| GSE103520_MCF7_gene_exp.diff.gz |
10.8 Mb |
(ftp)(http) |
DIFF |
| GSE103520_controls_gene_exp.diff.gz |
5.5 Mb |
(ftp)(http) |
DIFF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...