|
| Status |
Public on Aug 21, 2019 |
| Title |
Transformation of NIH3T3 cells by the forced expression of wild-type YAP or its stabilized form |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
YAP is known as a proto-oncogene with which the aberrant expression causes numerous types of cancer. To elucidate the molecular mechanisms underlying the acquisition of cancerous traits of normal cells by YAP overexpression, the wild-type YAP or its stabilized form was overexpressed in NIH3T3 cells. We found that the forced expression of either wild-type YAP or its derivative alone was sufficient for transforming NIH3T3 cells and we then analyzed the global expression profiles of the control NIH3T3 cells or those overexpressing wild-type or stabilized YAP.
|
| |
|
| Overall design |
The wild-type mouse Yap or the degradation resistant Yap in which serine at 112 was mutated by alanine was expressed in NIH3T3 cells.
|
| |
|
| Contributor(s) |
Nishimoto M, Hirasaki M, Mizuno Y, Okuda A |
| Citation(s) |
31358774 |
| |
| Submission date |
Sep 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Akihiko Okuda |
| E-mail(s) |
akiokuda@saitama-med.ac.jp
|
| Phone |
81-42-984-4787
|
| Organization name |
Saitama Medical University
|
| Department |
Research Center for Genomic Medicine
|
| Lab |
Division of Biomedical Sciences
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
3501241 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA401764 |
