|
| Status |
Public on Jan 12, 2018 |
| Title |
OMICS assisted N-terminal proteoform and protein expression profiling upon methionine aminopeptidase 1 (MetAP1) deletion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Excision of the N-terminal initiator methionine (iMet) residue from nascent peptide chains is an essential and omnipresent protein modification carried out by methionine aminopeptidases (MetAPs) that accounts for a major source of N-terminal proteoform diversity. While MetAP2 is known to be implicated in processes such as angiogenesis and proliferation in mammals, the physiological role of MetAP1 is much less clear. In this report we studied the omics-wide effects of human MetAP1 deletion and general MetAP inhibition. The levels of iMet retention are inversely correlated with cellular proliferation rates. Further, despite the increased MetAP2 expression observed upon MetAP1 deletion and as inferred from the iMet retention profiles observed, MetAP2 was unable to restore processing of MS-, MP- and MA- N-termini, indicating the higher activity of MetAP1 over these N-termini. Proteome and transcriptome expression profiling point to differential expression of proteins implicated in lipid metabolism, cytoskeleton organization, cell proliferation and protein synthesis upon perturbation of MetAP activity.
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| |
|
| Overall design |
RNA sequencing of wild type (parental) and METAP1 knockout HAP1 cells.
|
| |
|
| Contributor(s) |
Jonckheere V, Fijałkowska D, Van Damme P |
| Citation(s) |
29317475 |
| |
| Submission date |
Sep 01, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Petra Van Damme |
| E-mail(s) |
petra.vandamme@ugent.be
|
| Phone |
+3292649279
|
| Organization name |
VIB
|
| Street address |
Albert Baertsoenkaai 3
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (5)
|
| GSM2770692 |
wild type (parental) HAP1 cells biological replicate 1 |
| GSM2770693 |
wild type (parental) HAP1 cells biological replicate 2 |
| GSM2770694 |
wild type (parental) HAP1 cells biological replicate 3 |
| GSM2770695 |
METAP1 knockout HAP1 cells biological replicate 1 |
| GSM2770696 |
METAP1 knockout HAP1 cells biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA401719 |
| SRA |
SRP116953 |
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