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Series GSE103242 Query DataSets for GSE103242
Status Public on Dec 01, 2017
Title The transcriptome effect of overexpressing EZH2 in MCF7
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Increasing evidence suggests that epigenetic reprogramming contributes significantly to the development of endocrine therapy resistance in breast cancer. The goal of this work is to explore how the histone methyltransferase EZH2 interacts with ER signaling and drives the insensitiveness of breast cancer cells to the antagonistic effect of tamoxifen on ER activity. Therefore, we comprehensively analyzed the transcriptional program regulated by EZH2 in EZH2 overexpressed MCF-7 cells.
Methods: MCF-7 cells between passage 142-144 were used for this assay. For mRNA-Seq, cells are infected with control empty vector (EV) or EZH2 expressing plasmid (EZH2) by lentivirus. Total RNA were extracted by TRIzol (Invitrogen) and libraries were constructed using Illumina TruSeq RNA Sample Prep Kit v2 (Cat.# RS-122-2001). Hiseq 3000 was used for sequencing.
Overall design Transcriptome profiles of tamoxifen sensitive MCF-7 infected with pLenti-CMV-hygro-GFP or pLento-HA-EZH2 were sequenced in at least duplicates using Illumina HiSeq 3000.
Contributor(s) Wu Y, Zhang Z, Xu K
Citation(s) 29212856
Submission date Aug 29, 2017
Last update date Jul 25, 2021
Contact name Kexin Xu
Organization name UT Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl, MC 8257
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
GSM2758943 EV + Tamoxifen rep1
GSM2758944 EV + Tamoxifen rep2
GSM2758945 EV + Tamoxifen rep3
This SubSeries is part of SuperSeries:
GSE103243 Tamoxifen Resistance in Breast Cancer is Regulated by the EZH2-ERa-GREB1 Transcriptional Axis
BioProject PRJNA400606
SRA SRP116398

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103242_MCF7_overEZH2_readCounts.txt.gz 502.7 Kb (ftp)(http) TXT
GSE103242_RAW.tar 31.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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