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| Status |
Public on Aug 29, 2018 |
| Title |
Genome-wide regulatory architecture maps using ATAC-seq, H3K4me2 ChIP-seq, and PEAT in Drosophila S2 cells and ATAC-seq in whole worms |
| Organisms |
Caenorhabditis elegans; Drosophila melanogaster |
| Experiment type |
Other
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To assess the organization of regulatory architecture across organisms, we performed ATAC-seq to identify accessible regions of chromatin, H3K4me2 ChIP-seq to measure regulatory status, and PEAT to measure Transcription start sites of stable transcripts in Drosophila S2 cells. For comparison, ATAC-seq for accessible chromatin measurement was performed in whole stage L3 worms.
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| |
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| Overall design |
ATAC-seq, H3K4me2 ChIP-seq, and PEAT in Drosophila S2 cells and ATAC-seq in whole worms
|
| |
|
| Contributor(s) |
Lacadie S, Ohler U, Ibrahim M |
| Citation(s) |
30367057 |
| |
| Submission date |
Aug 28, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Scott Allen Lacadie |
| E-mail(s) |
scott.lacadie@mdc-berlin.de
|
| Organization name |
Max Delbrück Center for Molecular Medicine
|
| Department |
Berlin Institute for Medical Systems Biology
|
| Lab |
Ohler
|
| Street address |
Robert-Rössle-Str. 10
|
| City |
Berlin-Buch |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
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| Platforms (2) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA400453 |
| SRA |
SRP116321 |
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