|
| Status |
Public on Mar 21, 2018 |
| Title |
LPS stimulated murine RAW264.7 cells, incubated with DMSO carrier or mycolactone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Translational profiling of RAW264.7 cells stimulated with LPS in the presence and absence of 125ng/ml mycolactone: polysomal and subpolysomal RNA isolated by sucrose gradient centrifugation to determine the effect of mycolactone on translation during a proinflammatory response
|
| |
|
| Overall design |
Subpolysomal vs polysomal RNA under 2 conditions: control and mycolactone treated. Biological replicates: 3 replicates control, 3 replicates treated, with dyeswap
|
| |
|
| Contributor(s) |
Simmonds RE, Hall BS, Ogbechi J, Sbarrato T, Willis AE |
| Citation(s) |
29540678 |
| |
| Submission date |
Aug 23, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
RACHEL SIMMONDS |
| E-mail(s) |
rachel.simmonds@surrey.ac.uk
|
| Phone |
01483684714
|
| Organization name |
University of Surrey
|
| Street address |
FHMS UNIVERSITY OF SURREY, STAG HILL CAMPUS
|
| City |
GULDFORD |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
|
| Samples (6)
|
| GSM2752394 |
Control LPS-stimulated RAW264.7 cells Replicate1 |
| GSM2752395 |
Mycolactone treated LPS-stimulated RAW264.7 cells Replicate 1 |
| GSM2752396 |
Control LPS-stimulated RAW264.7 cells Replicate 2 |
| GSM2752397 |
Mycolactone treated LPS-stimulated RAW264.7 cells Replicate 2 |
| GSM2752398 |
Control LPS-stimulated RAW264.7 cells Replicate 3 |
| GSM2752399 |
Mycolactone treated LPS-stimulated RAW264.7 cells Replicate 3 |
|
| Relations |
| BioProject |
PRJNA399765 |
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