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Series GSE102965 Query DataSets for GSE102965
Status Public on Aug 24, 2017
Title Learned Helplessness Regulates Synapse Related LncRNAs in Mouse Hippocampus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: RNA sequencing (RNA-Seq) has revolutionized systems-based analysis of cellular pathways. The goal of this study is to find learned helplessness/depression regulates synapse related long noncoding RNAs (lncRNAs) in mouse hippocampus
Methods: compared the differential expression of mRNA and lncRNAs in learned helplessness (LH) group, nonlearned helplessness (NLH) group and Ctrl group, using GO analysis, pathway enrichment and co-expression
Results: We found that 2163 or 1396 mRNA are different expressed in LH compared with Control or NLH group, 199 or 142 lncRNAs are different expressed in LH compared with Control or NLH group, respectivly. Then we applied complex bioinfomatics analysis to do lncRNAs functional analysis, finding dendritic spine morphogenesis, chemical synaptic transmission and synaptic potential signaling pathway are common between LH group compared with Ctrl group and LH group compared with NLH group. Further more we found that the identified lncRNAs are mediating several cellular functions such as: protein transport, ion transport, mRNA processing, regulation of dendritic spine development, regulation of neuron projection development and regulation of GTPase activity. Interestingly, there was strong regulation of lncRNAs related to synapse.
Conclusions: Our study is the first attempt to explore the possibility that lncRNAs might be involved in helplessness using RNA sequencing. And we found some lncRNAs have strong functions in synapse regulation.
Overall design Hippocampus mRNA and lncRNAs profiles of LH/Ctrl and LH/NLH group mice were generated by RNA-Seq.
Contributor(s) Li C, Cao F
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Submission date Aug 23, 2017
Last update date May 15, 2019
Phone 0086-021-54237371
Organization name Fudan University
Department Institute of Brain Science and Department of Laboratory Animal Science
Street address 130 Dong An Road
City Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (16)
GSM2751718 Ctrl1
GSM2751719 Ctrl2
GSM2751720 Ctrl3
BioProject PRJNA399643
SRA SRP115985

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Supplementary file Size Download File type/resource
GSE102965_filter_isoform_fpkms.txt.gz 6.3 Mb (ftp)(http) TXT
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Processed data are available on Series record

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