|
| Status |
Public on May 11, 2018 |
| Title |
Sp8 and Sp9 Coordinately Promote Striatal Medium Spiny Neuron Production through Activating Six3 Expression [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To assess changes in gene expression profiles from the E16.5 no cre littermate control striatum and conditional double knockout of Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox mice.
Methods: Total RNA was isolated and sequenced from the striatum of the E16.5 no cre littermate control or Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox mice in duplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=2 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of Sp8 and Sp9.
Results: 80 genes were significantly increased and 120 genes were significantly decreased in expression level due to the loss of Sp8 and Sp9 expression.
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|
| Overall design |
Total RNA was isolated and sequenced from the striata of the E16.5 no cre littermate control or Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox mice in duplicate using an illumina high-seq 2500.
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| |
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| Contributor(s) |
Liang Q, Xu Z, Li Z, Wen Y, Liu G, Qi D, Zhang Z, Wang M, Wang C, Guo T, Song X, You Y, Chen B, Campbell K, Rubenstein JL, Yang Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Qifei Liang |
| E-mail(s) |
14111520020@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Street address |
138 Yi Xue Yuan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
| GSM2747376 |
mouse striatum_E16.5_control_no cre_rep1 |
| GSM2747377 |
mouse striatum_E16.5_control_no cre_rep2 |
| GSM2747378 |
mouse striatum_E16.5_control_no cre_rep3 |
| GSM2747379 |
mouse striatum_E16.5_Sp8/Sp9-DCKO_Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox_rep1 |
| GSM2747380 |
mouse striatum_E16.5_Sp8/Sp9-DCKO_Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox_rep2 |
| GSM2747381 |
mouse striatum_E16.5_Sp8/Sp9-DCKO_Dlx5/6-CIE; Sp8 Flox/Flox; Sp9 Flox/Flox_rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE114344 |
Sp8 and Sp9 Coordinately Promote Striatal Medium Spiny Neuron Production through Activating Six3 Expression |
|
| Relations |
| BioProject |
PRJNA399136 |
| SRA |
SRP115902 |
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