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| Status |
Public on Aug 19, 2017 |
| Title |
Expression profiling macrophage subsets [P2538] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Experiments expression profile several FACS-sorted pancreas tumor-associated macrophages.
Tumor-associated macrophages (TAMs) are essential components of the cancer microenvironment and play critical roles in the regulation of tumor progression. Optimal therapeutic intervention requires in-depth understanding of the sources that sustain macrophages in malignant tissues. In this study, we investigated the ontogeny of TAMs in murine pancreatic ductal adenocarcinoma (PDAC) models. We identified both inflammatory monocytes and tissue-resident macrophages as sources of TAMs. Unexpectedly, significant portions of pancreas-resident macrophages originate during embryonic development and expand through in situ proliferation during tumor progression. While monocyte-derived TAMs play more potent roles in antigen presentation, embryonically derived TAMs exhibit a pro-fibrotic transcriptional profile, indicative of their role in producing and remodeling extracellular matrix molecules. Collectively, these findings uncover the heterogeneity of TAM origin and functions, and could provide therapeutic insight for PDAC treatment.
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| Overall design |
Macrophages were either sorted from the normal pancreas or PDAC tissues of wild-type, or KPC or FLT3-CRE/LSL-YFP mice.
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| Contributor(s) |
Zhu Y, Knolhoff B, DeNardo D |
| Citation(s) |
28930665 |
| |
| Submission date |
Aug 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
DAvid DeNardo |
| E-mail(s) |
ddenardo@wustl.edu
|
| Organization name |
Washington University
|
| Department |
Medicine
|
| Street address |
660 S. Euclid Ave
|
| City |
St. Louis |
| State/province |
Mo |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE102836 |
Expression profiling macrophage subsets |
|
| Relations |
| BioProject |
PRJNA399232 |
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