|
| Status |
Public on Aug 26, 2008 |
| Title |
Khd1p associated mRNAs |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
RNA-coimmunopurifications with TAP-tagged Khd1 protein from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control (Gerber et al., 2004). Cells were grown to midlog phase in rich media and harvested by centrifugation. TAP-tagged Khd1 was affinity purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input) and from purified protein samples by phenol-chloroform extraction. RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/ dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast DNA microarrays over night at 65 degrees. For a detailed procedure see http://microarray-pubs.stanford.edu/yeast_puf and also Gerber AP et al. PLoS Biology, 2004.
An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
Strain Name: yeast strain with/without TAP-tagged Khd1
Keywords: all_pairs
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| |
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| Overall design |
Computed
|
| |
|
| Contributor(s) |
Gerber A |
| Citation(s) |
18805955 |
| |
| Submission date |
Jan 25, 2008 |
| Last update date |
Jul 29, 2013 |
| Contact name |
Andre Gerber |
| E-mail(s) |
andre.gerber@pharma.ethz.ch
|
| Phone |
+41 44 6337378
|
| Fax |
+41 44 6337364
|
| Organization name |
Swiss Federal Institute of Technology
|
| Department |
Institute of Pharmaceutical Science
|
| Street address |
Wolfgang Pauli Str. 10, HCI H396
|
| City |
ETH Hoenggerberg, Zuerich |
| ZIP/Postal code |
ZH |
| Country |
Switzerland |
| |
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| Platforms (3) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA108479 |
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