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| Status |
Public on May 31, 2018 |
| Title |
Genome wide transcriptome analysis of Jak2 KD persistent murine Eμ-Crlf2/Jak2R683G B-ALL cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq was performed to compare the transcriptional programmes of murine Eµ-Crlf2/Jak2R683G-expressing B-ALL cells subjected to short-term (2 days) versus long-term (21 days) shRNA-mediated Jak2 knockdown in vivo.
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| Overall design |
NSG recipient mice of Eµ-Crlf2/Jak2R683G-pREBIR-shRenilla.713 or Eµ-Crlf2/Jak2R683G–pREBIR-shJak2.3323 cells were sacrificed at either 48 hr (acute knockdown) or 21 days (chronic knockdown) post shRNA induction and RNA-Seq was performed using total RNA isolated from dsRed+/eBFP2+ splenocytes. Experiment was performed in triplicate (three independent mice).
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| Contributor(s) |
Kim S, Johnstone R |
| Citation(s) |
29907650 |
| |
| Submission date |
Aug 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
SANGKYU KIM |
| E-mail(s) |
sang-kyu.kim@petermac.org
|
| Organization name |
PETER MACCALLUM CANCER CENTRE
|
| Department |
RESEARCH
|
| Lab |
GENE REGULATION
|
| Street address |
305 GRATTAN ST
|
| City |
MELBOURNE |
| State/province |
VICTORIA |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE102535 |
JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias |
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| Relations |
| BioProject |
PRJNA397985 |
| SRA |
SRP115269 |
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