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| Status |
Public on Aug 10, 2020 |
| Title |
Transcriptome profiling of in vitro cellular entities involved in lung tumour fusion events |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cell fusion theory for explaining metastasis origin has been hypothesised for almost one-hundred years, mainly availed with data coming from patients who received a bone marrow transplant and all had tumour cells with DNA from their cancer-free donors. In fact, colonisation of distant organs by an original tumour could be explained with the acquisition of hallmarks from both immune and tumour parents. Here, we systematically conducted in vitro fusions between CD14+human monocytes and GFP+H60 lung tumour cells. Transcriptome analysis of naïve monocytes, and sorted GFP+, CD14+ and CD14+GFP+ cells showed the latter sharing common pathways with both parental types, which sustain their in vivo metastatic characteristics, such as mobility and invasiveness. Altogether, our data demonstrates that differentially genes profile acquisition by hybrids enables them to drive metastasis.
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| Overall design |
RNA-seq in biological replicates of four days in vitro fusions between human PBMC derived monocytes and GFP+CSC-H460 tumour cell line.
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| Contributor(s) |
López-Collazo E, Aguirre LA, Schulte LN |
| Citation |
Luis Augusto Aguirre, Karla Montalbán-Hernández, José Avendaño-Ortiz, Elvira Marín, Roberto Lozano, Víctor Toledano, Laura Sánchez-Maroto, Verónica Terrón, Jaime Valentín, Elisa Pulido, José Carlos Casalvilla, Carolina Rubio, Luke Diekhorst, Fernando Laso-García, Carlos del Fresno, Ana Collazo-Lorduy, Beatriz Jiménez-Munarriz, Paloma Gómez-Campelo, Emilio Llanos-González, María Fernández-Velasco, Carlos Rodríguez-Antolín, Rebeca Pérez de Diego, Ramón Cantero-Cid, Enrique Hernádez-Jimenez, Enrique Álvarez, Rocío Rosas, Blanca dies López-Ayllón, Javier de Castro, Stefanie K. Wculek, Carolina Cubillos-Zapata, Inmaculada Ibáñez de Cáceres, Prudencio Díaz-Agero, María Gutiérrez Fernández, María Paz de Miguel, David Sancho, Leon Schulte, Rosario Perona, Cristóbal Belda-Iniesta, Lisardo Boscá & Eduardo López-Collazo. Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells. OncoImmunology 2020;9:1. doi:10.1080/2162402X.2020.1773204
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| Submission date |
Aug 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Leon Nicolas Schulte |
| E-mail(s) |
leon.schulte@uni-marburg.de
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| Organization name |
Philipps-Universität Marburg
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| Department |
Institute for Lung Research
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| Lab |
Schulte
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| Street address |
Hans-Meerwein-Str. 2
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| City |
Marburg |
| State/province |
Hessen |
| ZIP/Postal code |
35043 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA397926 |
| SRA |
SRP115235 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102512_RAW.tar |
16.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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