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GEO help: Mouse over screen elements for information. |
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Status |
Public on Mar 31, 2008 |
Title |
Complement activation in the peripheral nervous system following the spinal nerve ligation model of neuropathic pain |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Neuroinflammatory and neuroimmune mechanisms, as exemplified by infiltrating immune cells and activation of resident endothelial/glial cells, respectively, are known to be involved in the establishment and maintenance of chronic pain. An immune system pathway that may be involved in the activation of both immune and glial cells is complement. The complement pathway is made up of a large number of distinct plasma proteins which react with one another to opsonize pathogens and induce a series of inflammatory responses to help fight infection. Cleaved products and complexes produced by complement activation are responsible for a range of effects including mediation of immune infiltration, activation of phagocytes, opsonization/lysis of pathogens and injured cells, and production of vasoactive amines such as histamine and serotonin. Gene-expression microarray-analysis was performed on the rat spinal nerve ligation (SNL) model of neuropathic pain Keywords: organ type comparison, disease state analysis and drug treatment
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Overall design |
For gene expression analysis, rats from five experimental groups were sacrificed 19–21 days after surgery: (1)Naïve+ vehicle; (2) Naïve + GPN; (3) sham + vehicle; (4) SNL + vehicle; (5) SNL + GPN. Tissue was collected from whole brain, hemisected spinal cord, mid thigh sciatic nerve, and L4, L5 and L6 dorsal root ganglia (DRG), both ipsilateral(ipsi) and contralateral (contra) to the injury. In addition,‘‘organ recital’’ tissues were harvested from naı ¨ve animals: adrenal,aorta, fetal brain, kidney, liver, quadriceps muscle, spleen,submaxillary gland, and testis and 15 other tissues.For each experimental group and tissue, samples rapidly frozen on dry ice were separated into two pools(Pool 1 and Pool 2), consisting of half or 4-6 animals each.Pool 1 total RNA was subjected to standard methods on Affymetrix GeneChip Arrays using rat U34 A, B, and C.
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Contributor(s) |
Levin ME, Jin JG, Ji R, Tong J, Pomonis JD, Lavery DJ, Miller SW, Chiang LW |
Citation(s) |
18160218 |
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Submission date |
Jan 22, 2008 |
Last update date |
Feb 21, 2017 |
Contact name |
Jason Gang Jin |
E-mail(s) |
jasongjin@gmail.com
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Phone |
732-220-9988
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Fax |
732-960-2355
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Organization name |
MaxyBio
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Street address |
675 US Highway
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City |
North Brunswick |
ZIP/Postal code |
08902 |
Country |
USA |
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Platforms (3) |
GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
GPL86 |
[RG_U34B] Affymetrix Rat Genome U34 Array |
GPL87 |
[RG_U34C] Affymetrix Rat Genome U34 Array |
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Samples (156)
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GSM258322 |
Naive nerve,Ipsi set A |
GSM258323 |
Naive sc,Ipsi set A |
GSM258324 |
Naive L4 DRG,Contra set A |
GSM258325 |
Naive L5 DRG,Contra set A |
GSM258326 |
Naive nerve,contra set A |
GSM258327 |
Naive sc, contra set A |
GSM258328 |
Naive brain set A |
GSM258329 |
Naive+ GPN L4 DRG,Ipsi set A |
GSM258330 |
Naive+ GPN L5 DRG,Ipsi set A |
GSM258331 |
Naive+ GPN L6 DRG,Ipsi set A |
GSM258332 |
Naive+ GPN nerve,Ipsi set A |
GSM258333 |
Naive+ GPN sc,Ipsi set A |
GSM258334 |
Naive+ GPN L4 DRG,Contra set A |
GSM258335 |
Naive+ GPN L6 DRG,Contra set A |
GSM258336 |
Naive+ GPN nerve,contra set A |
GSM258337 |
Naive+ GPN sc, contra set A |
GSM258338 |
Naive+ GPN brain set A |
GSM258339 |
Sham L4 DRG, Ipsi set A |
GSM258340 |
Sham L5 DRG,Ipsi set A |
GSM258341 |
Sham L6 DRG,Ipsi set A |
GSM258342 |
Sham nerve,Ipsi set A |
GSM258343 |
Sham sc,Ipsi set A |
GSM258344 |
Sham L4 DRG,Contra set A |
GSM258345 |
Sham L5 DRG,Contra set A |
GSM258346 |
Sham L6 DRG,Contra set A |
GSM258347 |
Sham nerve,contra set A |
GSM258348 |
Sham sc, contra set A |
GSM258349 |
Sham brain set A |
GSM258350 |
SNL L4 DRG, Ipsi set A |
GSM258351 |
SNL L5 DRG,Ipsi set A |
GSM258352 |
SNL L6 DRG,Ipsi set A |
GSM258353 |
SNL nerve,Ipsi set A |
GSM258354 |
SNL sc,Ipsi set A |
GSM258355 |
SNL L4 DRG,Contra set A |
GSM258356 |
SNL L5 DRG,Contra set A |
GSM258357 |
SNL L6 DRG,Contra set A |
GSM258358 |
SNL sc, contra set A |
GSM258359 |
SNL brain set A |
GSM258360 |
SNL+ GPN L4 DRG,Ipsi set A |
GSM258361 |
SNL+ GPN L5 DRG,Ipsi set A |
GSM258362 |
SNL+ GPN L6 DRG,Ipsi set A |
GSM258363 |
SNL+ GPN nerve,Ipsi set A |
GSM258364 |
SNL+ GPN sc,Ipsi set A |
GSM258365 |
SNL+ GPN L4 DRG,Contra set A |
GSM258366 |
SNL+ GPN L5 DRG,Contra set A |
GSM258367 |
SNL+ GPN L6 DRG,Contra set A |
GSM258368 |
SNL+ GPN nerve,contra set A |
GSM258369 |
SNL+ GPN sc, contra set A |
GSM258370 |
SNL+ GPN brain set A |
GSM258371 |
Naive L4 DRG,Ipsi set B |
GSM258372 |
Naive L5 DRG,Ipsi set B |
GSM258373 |
Naive L6 DRG,Ipsi set B |
GSM258374 |
Naive nerve,Ipsi set B |
GSM258375 |
Naive sc,Ipsi set B |
GSM258376 |
Naive L4 DRG,Contra set B |
GSM258377 |
Naive L5 DRG,Contra set B |
GSM258378 |
Naive nerve,contra set B |
GSM258379 |
Naive sc, contra set B |
GSM258380 |
Naive brain set B |
GSM258381 |
Naive+ GPN L4 DRG,Ipsi set B |
GSM258382 |
Naive+ GPN L5 DRG,Ipsi set B |
GSM258383 |
Naive+ GPN L6 DRG,Ipsi set B |
GSM258384 |
Naive+ GPN nerve,Ipsi set B |
GSM258385 |
Naive+ GPN sc,Ipsi set B |
GSM258386 |
Naive+ GPN L4 DRG,Contra set B |
GSM258387 |
Naive+ GPN L6 DRG,Contra set B |
GSM258388 |
Naive+ GPN nerve,contra set B |
GSM258389 |
Naive+ GPN sc, contra set B |
GSM258390 |
Naive+ GPN brain set B |
GSM258391 |
Sham L4 DRG, Ipsi set B |
GSM258392 |
Sham L5 DRG,Ipsi set B |
GSM258393 |
Sham L6 DRG,Ipsi set B |
GSM258394 |
Sham nerve,Ipsi set B |
GSM258395 |
Sham sc,Ipsi set B |
GSM258396 |
Sham L4 DRG,Contra set B |
GSM258397 |
Sham L5 DRG,Contra set B |
GSM258398 |
Sham L6 DRG,Contra set B |
GSM258399 |
Sham nerve,contra set B |
GSM258400 |
Sham sc, contra set B |
GSM258401 |
Sham brain set B |
GSM258402 |
SNL L4 DRG, Ipsi set B |
GSM258403 |
SNL L5 DRG,Ipsi set B |
GSM258404 |
SNL L6 DRG,Ipsi set B |
GSM258405 |
SNL nerve,Ipsi set B |
GSM258406 |
SNL sc,Ipsi set B |
GSM258407 |
SNL L4 DRG,Contra set B |
GSM258408 |
SNL L5 DRG,Contra set B |
GSM258409 |
SNL L6 DRG,Contra set B |
GSM258410 |
SNL sc, contra set B |
GSM258411 |
SNL brain set B |
GSM258412 |
SNL+ GPN L4 DRG,Ipsi set B |
GSM258413 |
SNL+ GPN L5 DRG,Ipsi set B |
GSM258414 |
SNL+ GPN L6 DRG,Ipsi set B |
GSM258415 |
SNL+ GPN nerve,Ipsi set B |
GSM258416 |
SNL+ GPN sc,Ipsi set B |
GSM258417 |
SNL+ GPN L4 DRG,Contra set B |
GSM258418 |
SNL+ GPN L5 DRG,Contra set B |
GSM258419 |
SNL+ GPN L6 DRG,Contra set B |
GSM258420 |
SNL+ GPN nerve,contra set B |
GSM258421 |
SNL+ GPN sc, contra set B |
GSM258422 |
SNL+ GPN brain set B |
GSM258423 |
Naive L4 DRG,Ipsi set C |
GSM258424 |
Naive L5 DRG,Ipsi set C |
GSM258425 |
Naive L6 DRG,Ipsi set C |
GSM258426 |
Naive nerve,Ipsi set C |
GSM258427 |
Naive sc,Ipsi set C |
GSM258428 |
Naive L4 DRG,Contra set C |
GSM258429 |
Naive L5 DRG,Contra set C |
GSM258430 |
Naive nerve,contra set C |
GSM258431 |
Naive sc, contra set C |
GSM258432 |
Naive brain set C |
GSM258433 |
Naive+ GPN L4 DRG,Ipsi set C |
GSM258434 |
Naive+ GPN L5 DRG,Ipsi set C |
GSM258435 |
Naive+ GPN L6 DRG,Ipsi set C |
GSM258436 |
Naive+ GPN nerve,Ipsi set C |
GSM258437 |
Naive+ GPN sc,Ipsi set C |
GSM258438 |
Naive+ GPN L4 DRG,Contra set C |
GSM258439 |
Naive+ GPN L6 DRG,Contra set C |
GSM258440 |
Naive+ GPN nerve,contra set C |
GSM258441 |
Naive+ GPN sc, contra set C |
GSM258442 |
Naive+ GPN brain set C |
GSM258443 |
Sham L4 DRG, Ipsi set C |
GSM258444 |
Sham L5 DRG,Ipsi set C |
GSM258445 |
Sham L6 DRG,Ipsi set C |
GSM258446 |
Sham nerve,Ipsi set C |
GSM258447 |
Sham sc,Ipsi set C |
GSM258448 |
Sham L4 DRG,Contra set C |
GSM258449 |
Sham L5 DRG,Contra set C |
GSM258450 |
Sham L6 DRG,Contra set C |
GSM258451 |
Sham nerve,contra set C |
GSM258452 |
Sham sc, contra set C |
GSM258453 |
Sham brain set C |
GSM258454 |
SNL L4 DRG, Ipsi set C |
GSM258455 |
SNL L5 DRG,Ipsi set C |
GSM258456 |
SNL L6 DRG,Ipsi set C |
GSM258457 |
SNL nerve,Ipsi set C |
GSM258458 |
SNL sc,Ipsi set C |
GSM258459 |
SNL L4 DRG,Contra set C |
GSM258460 |
SNL L5 DRG,Contra set C |
GSM258461 |
SNL L6 DRG,Contra set C |
GSM258462 |
SNL sc, contra set C |
GSM258463 |
SNL brain set C |
GSM258464 |
SNL+ GPN L4 DRG,Ipsi set C |
GSM258465 |
SNL+ GPN L5 DRG,Ipsi set C |
GSM258466 |
SNL+ GPN L6 DRG,Ipsi set C |
GSM258467 |
SNL+ GPN nerve,Ipsi set C |
GSM258468 |
SNL+ GPN sc,Ipsi set C |
GSM258469 |
SNL+ GPN L4 DRG,Contra set C |
GSM258470 |
SNL+ GPN L5 DRG,Contra set C |
GSM258471 |
SNL+ GPN L6 DRG,Contra set C |
GSM258472 |
SNL+ GPN nerve,contra set C |
GSM258473 |
SNL+ GPN sc, contra set C |
GSM258474 |
SNL+ GPN brain set C |
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Relations |
BioProject |
PRJNA108415 |
Supplementary file |
Size |
Download |
File type/resource |
GSE10238_RAW.tar |
283.8 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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