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| Status |
Public on Aug 04, 2019 |
| Title |
SUN protein regulates transcription-associated epigenetic modification |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the difference of genome-wide chromatin profiling of histone modifications in mammalian cells after PBS or LPS treatment. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse peritoneal macrophages. We find that lysine 4 and lysine 9 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential.Trimethylation of lysine 9 is detected at satellite, telomeric and active long-terminal repeats, and can spread into proximal unique sequences. Lysine 4 and lysine 9 trimethylation marks imprinting control regions. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of mammalian cells after LPS treatment.
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| Overall design |
Examination of 2 different histone modifications in mouse peritoneal macrophages after LPS treatment.
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| Contributor(s) |
Jiao S, Li C |
| Citation missing |
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| |
| Submission date |
Aug 04, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
jingmin Guan |
| E-mail(s) |
jmguan@sibs.ac.cn
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| Organization name |
Shanghai Institutes for Biological Sciences
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| Department |
Institute of Biochemistry and Cell Biolog
|
| Street address |
320 Yue Yang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA397136 |
| SRA |
SRP114825 |
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