|
| Status |
Public on Nov 12, 2018 |
| Title |
Ribosome profiling analysis of heat effect on rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
To impose the heat treatment similar to the Structure-seq experiment, we applied Ribo-seq to 14-day-old rice (Oryza sativa) shoot tissue to investigate the effect of heat stress (42°C) as compared to control condition (22°C).
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| Overall design |
Two-week-old rice plants in pots were inverted and the shoots were immersed in a water bath at 22°C or 42°C for 10 min treatment, and ten shoots were harvested for each individual sample. Two biological replicates were obtained for each treatment and time-point. Ribosome profiling libraries were then made using these four sets of materials to determine the translational efficiency changes under different temperatures.
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| Contributor(s) |
Su Z, Tang Y, Bevilacqua P, Assmann SM |
| Citation(s) |
30413617 |
| |
| Submission date |
Aug 03, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhao SU |
| Organization name |
The Penn State University
|
| Department |
Biology
|
| Lab |
Sarah Assmann
|
| Street address |
354 N. Frear
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19290 |
Illumina HiSeq 2500 (Oryza sativa) |
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| Samples (4)
|
| GSM2730475 |
C10m_rice_22oC_10min_riboseq_rep1.fastq.gz |
| GSM2730476 |
C10m_rice_22oC_10min_riboseq_rep2.fastq.gz |
| GSM2730477 |
H10m_rice_42oC_10min_riboseq_rep1.fastq.gz |
| GSM2730478 |
H10m_rice_42oC_10min_riboseq_rep2.fastq.gz |
|
| Relations |
| BioProject |
PRJNA397035 |
| SRA |
SRP114761 |
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