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| Status |
Public on Jul 20, 2018 |
| Title |
lncRNA and mRNA-sequencing of WS1 cells after 5Gy of X-Ray irradiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goal of this study was to compare the lncRNA and mRNA expression profile of human fibroblast WS1 cells between 0 Gy and 5 Gy X-ray irradiation.
Methods: lncRNA and mRNA-sequencing of WS1 cells were generated using Illuminal Hiseq4000, RNA-seq reads were mapped to human genome hg19 by hisat2 software. Cuffdiff software was used to analysis the differentially expressed levels of LncRNA and mRNA FPKM.
Results: We identified different expressed levels of 88780 lnRNA and 20311 mRNA transcripts in WS1 cells between 0 Gy and 5 Gy X-ay irradiation.
Conclusion: Our study first revealed the detail of lncRNA and mRNA expression profiles of WS1 cells after 5Gy of X-Ray irradiation
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| Overall design |
lncRNA and mRNA-sequencing of WS1 cells were generated using Illuminal Hiseq4000, RNA-seq reads were mapped to human genome hg19 by hisat2 software. Cuffdiff software was used to analysis the differentially expressed levels of LncRNA and mRNA FPKM.
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| Contributor(s) |
Zhang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
shuyu zhang |
| E-mail(s) |
mathhavs@hotmail.com
|
| Phone |
15851417273
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| Organization name |
Soochow University
|
| Street address |
199 Ren'ai Road
|
| City |
Suzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE102147 |
High throughput sequencing of human fibroblast WS1 cells after 5 Gy X-ray irradiation |
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| Relations |
| BioProject |
PRJNA396832 |
| SRA |
SRP114660 |
