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| Status |
Public on Dec 04, 2018 |
| Title |
Profiling proliferative cells and their progeny in damaged murine hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The significance of cardiac stem cell (CSC) populations for cardiac regeneration remains disputed. Here, we apply the most direct definition of stem cell function (the ability to replace lost tissue through cell division) to interrogate the existence of CSCs. By single-cell mRNA sequencing and genetic lineage tracing using two Ki67 knockin mouse models, we map all proliferating cells and their progeny in homoeostatic and regenerating murine hearts. Cycling cardiomyocytes were only robustly observed in the early postnatal growth phase, while cycling cells in homoeostatic and damaged adult myocardium represented various noncardiomyocyte cell types. Proliferative postdamage fibroblasts expressing follistatin-like protein 1 (FSTL1) closely resemble neonatal cardiac fibroblasts and form the fibrotic scar. Genetic deletion of Fstl1 in cardiac fibroblasts results in postdamage cardiac rupture. We find no evidence for the existence of a quiescent CSC population, for transdifferentiation of other cell types toward cardiomyocytes, or for proliferation of significant numbers of cardiomyocytes in response to cardiac injury.
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| Overall design |
We generated transciptome data from proliferative cardiac cells collected from 3, 7 or 14 days following myocardial infarction (MI) or sham surgery.
This series includes single-cell transcriptome data from (Ki67-RFP+) cardiac cells collected from neonatal murine hearts, adult homeostatic murine hearts or adult murine hearts collected 14 days following myocardial infarction (MI), ischemic/perfusion (I/R) or sham surgery.
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| Web link |
https://www.pnas.org/content/115/52/E12245
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| Contributor(s) |
Kretzschmar K, Post Y, Bannier-Hélaouët M, Drost J, Basak O, Li VS, van den Born M, Versteeg D, Kooijman L, van der Elst S, van Es JH, van Rooij E, Clevers H |
| Citation(s) |
30530645 |
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| Submission date |
Jul 31, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Kai Kretzschmar |
| E-mail(s) |
kai.kretzschmar@uni-wuerzburg.de
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| Organization name |
University Hospital Würzburg
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| Street address |
Josef-Schneider-Str. 2
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (63)
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| GSM2722416 |
Extracted RNA from Ki67-RFP+ cells of hearts of Ki67TagRFP mice 14 days post-MI surgery, replicate 1_bulk RNA |
| GSM2722417 |
Extracted RNA from Ki67-RFP+ cells of hearts of Ki67TagRFP mice 14 days post-MI surgery, replicate 2_bulk RNA |
| GSM2722418 |
Extracted RNA from Ki67-RFP+ cells of hearts of Ki67TagRFP mice 14 days post-SHAM surgery, replicate 1_bulk RNA |
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| Relations |
| BioProject |
PRJNA396493 |
| SRA |
SRP114373 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102048_CEL-Seq2_UMI8_barcodes.csv.gz |
126 b |
(ftp)(http) |
CSV |
| GSE102048_CEL-Seq2_UMI_barcodes.csv.gz |
2.1 Kb |
(ftp)(http) |
CSV |
| GSE102048_CEL-Seq_UMI_barcodes.csv.gz |
429 b |
(ftp)(http) |
CSV |
| GSE102048_Ki67-RFP_bulk-seq_mapped_data.csv.gz |
147.5 Kb |
(ftp)(http) |
CSV |
| GSE102048_Ki67-RFP_bulk-seq_ndata.csv.gz |
160.4 Kb |
(ftp)(http) |
CSV |
| GSE102048_RAW.tar |
15.2 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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