|
| Status |
Public on Oct 30, 2017 |
| Title |
Gene expression profiling of murine skin transgenically generated to express HPV16 E7 oncoprotein |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A validated animal model would assist with research on the immunological consequences of the chronic expression of HPV16 E7 oncoprotein expression. Here we performed RNA-seq analysis of normal C57BL/6 skin versus mouse transgenically generated to express HPV16 E7 oncogene under the keratin 14 promoter (K14E7). Mice present with epithelial hyperplasia and increased immune cell infiltrate associated with immune suppression.
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| |
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| Overall design |
Ear skin (n=6) 8-week old C57BL/6 and K14E7 mice
Grafted samples (n=18) of donor C57BL/6, K14E7 and CD1d-/-xK14E7 skin on C57BL6 recipients
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| |
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| Contributor(s) |
Tuong ZK, Frazer IH |
| Citation(s) |
27333029 |
|
ZK Tuong, K Noske, P Kuo, AA Bashaw, SM Teoh, IH Frazer. Murine HPV16 E7-expressing transgenic skin effectively emulates the cellular and molecular features of human high-grade squamous intraepithelial lesions. Papillomavirus Research 2017. doi:10.1016/j.pvr.2017.10.001
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| BioProject |
PRJNA395772 |
| |
| Submission date |
Jul 28, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Zewen Kelvin Tuong |
| E-mail(s) |
z.tuong@uq.edu.au
|
| Organization name |
The University of Queensland
|
| Street address |
62 Graham Street, Centre for Children's Health Research (CCHR), South Brisbane, QLD, 4101
|
| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4101 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (24)
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| Relations |
| SRA |
SRP113560 |
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