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| Status |
Public on Dec 13, 2017 |
| Title |
RNAseq of CD45+ cells excluding mast cells from the skin of K14HPV16 Mcpt5-Cre- and K14HPV16 Mcpt5-Cre+ mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The experiment aimed at determing the influence of mast cell deficiency on the transcriptome of skin-infiltrating leukocytes in K14HPV16 mice at 2month and 6month of age.
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| Overall design |
Skin-inflitrating leucocytes were FACS-purified from mast cell proficient (Mcpt5-Cre-) and mast cell deficient (Mcpt5-Cre+) K14HPV16 mice. Mast cells (CD117 high, FCeR1 high) were excluded from the sorting gate. In order to control for minimal mast cell contamination during sorting from K14HPV16 Mcpt5-Cre- skin, mast cell signature transcripts were identified by comparing transcriptomes of samples fromK14HPV16 Mcpt5-Cre- mice in which mast cells were flow cytometrically included vs excluded.
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| Contributor(s) |
Roers A, Ghouse SM, Behrendt R |
| Citation(s) |
29298428 |
| |
| Submission date |
Jul 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Axel Roers |
| E-mail(s) |
Axel.Roers@med.uni-heidelberg.de
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| Organization name |
Heidelberg University Hospital
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| Department |
Institute of Immunology
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| Lab |
Roers Lab
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| Street address |
Im Neuenheimer Feld 305
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (19)
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| This SubSeries is part of SuperSeries: |
| GSE101997 |
Although abundant in tumor tissue, mast cells have no effect on immunological micro-milieu or growth of HPV-induced or transplanted tumors. |
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| Relations |
| BioProject |
PRJNA396164 |
| SRA |
SRP113763 |
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