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| Status |
Public on Oct 15, 2018 |
| Title |
H3K79me2 ChIP-seq in cerebellar granular neurons (CGN) and progenitors (CGNP) upon DOT1L inhibition with SGC0946 in vitro |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
DOT1L as methyltransferase of H3K79 is implicated in brian development. Here, we further defined DOT1L function within the granular neurons during cerebellar development using ChIP-seq of H3K79 dimethylation of isolated cerebellar granular neurons and progenitors. Thereby we compared samples treated with a DOT1L inhibitor versus DMSO treated cells. The data sets reveals new important targets of DOT1L, which ensure a correct development of the cerebellum.
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| Overall design |
H3K79me2 ChIP-seq of CGNPs,4 h after DOT1L inhibition and CGNs, 44 h after DOT1L inhibition; compared to DMSO control each. Cells derived from murine cerebellum of P7 NMRI WT mice
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| Contributor(s) |
Bovio P, Kilpert F, Bönisch U, Manke T, Vogel T |
| Citation(s) |
30302725 |
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| Submission date |
Jul 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Patrick Piero Bovio |
| Organization name |
Albert-Ludwigs University Freiburg, Institute of Anatomy and Cell Biology
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| Department |
Department of Molecular Embryology
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| Lab |
Vogel
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| Street address |
Albertstr. 19
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| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (16)
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| GSM2719369 |
Input, Mus Musculus CGNP DMSO , biological replicate 1 |
| GSM2719370 |
Input, Mus Musculus CGNP DMSO, biological replicate 2 |
| GSM2719371 |
H3K79me2, ChIPseq, Mus Musculus CGNP DMSO, biological replicate 1 |
| GSM2719372 |
H3K79me2, ChIPseq, Mus Musculus CGNP DMSO, biological replicate 2 |
| GSM2719373 |
Input, Mus Musculus CGNP SGC0946 , biological replicate 1 |
| GSM2719374 |
Input, Mus Musculus CGNP SGC0946, biological replicate 2 |
| GSM2719375 |
H3K79me2, ChIPseq, Mus Musculus CGNP SGC0946, biological replicate 1 |
| GSM2719376 |
H3K79me2, ChIPseq, Mus Musculus CGNP SGC0946, biological replicate 2 |
| GSM2719377 |
Input, Mus Musculus CGN DMSO , biological replicate 1 |
| GSM2719378 |
Input, Mus Musculus CGN DMSO, biological replicate 2 |
| GSM2719379 |
H3K79me2, ChIPseq, Mus Musculus CGN DMSO, biological replicate 1 |
| GSM2719380 |
H3K79me2, ChIPseq, Mus Musculus CGN DMSO, biological replicate 2 |
| GSM2719381 |
Input, Mus Musculus CGN SGC0946 , biological replicate 1 |
| GSM2719382 |
Input, Mus Musculus CGN SGC0946, biological replicate 2 |
| GSM2719383 |
H3K79me2, ChIPseq, Mus Musculus CGN SGC0946, biological replicate 1 |
| GSM2719384 |
H3K79me2, ChIPseq, Mus Musculus CGN SGC0946, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE101949 |
Cerebellar granular neurons (CGN) and progenitors (CGNP) upon DOT1L inhibition or cKO |
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| Relations |
| BioProject |
PRJNA395990 |
| SRA |
SRP113674 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE101947_RAW.tar |
3.0 Gb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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