NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE101732 Query DataSets for GSE101732
Status Public on Jul 22, 2017
Title RNA-sequencing of Mbd3-null and control haematopoietic stem cell and lymphoid progenitor cell populations
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the role of Mbd3/NuRD in lymphopoiesis, gene expression in purified populations of Mbd3-deleted and control lymphoid progenitor cells was analysed using RNA-seq.
 
Overall design Mbd3-deficient and control lymphoid progenitors were isolated from mouse bone marrow by flow cytometry, including haematopoietic stem cells (HSCs), lymphoid-primed multipotent progenitors (LMPPs), all-lymphoid progenitors (ALPs) and B cell-biased lymphoid progenitors (BLPs). RNA-seq was performed on 100 HSCs or 150 cells from the other populuations, using the previously described smartseq2 protocol for RNA-seq of small numbers of cells (Picelli et al. (2014) Nature protocols 9:171).
 
Contributor(s) Loughran SJ
Citation(s) 28899870
Submission date Jul 21, 2017
Last update date May 15, 2019
Contact name Stephen John Loughran
E-mail(s) stephenloughran@gmail.com
Organization name University of Cambridge
Department Haematology
Street address Wellcome Trust/MRC building, Addenbrooke's Hospital
City Cambridge
ZIP/Postal code CB2 0XY
Country United Kingdom
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (40)
GSM2714172 RNA-seq of Mus musculus : Mbd3-floxed all-lymphoid progenitor cells (ALP) [control_ALP_mouse10357]
GSM2714173 RNA-seq of Mus musculus : Mbd3-floxed all-lymphoid progenitor cells (ALP) [control_ALP_mouse10358]
GSM2714174 RNA-seq of Mus musculus : Mbd3-floxed all-lymphoid progenitor cells (ALP) [control_ALP_mouse10372]
This SubSeries is part of SuperSeries:
GSE101735 Mbd3-null and control lymphoid progenitor cells
Relations
BioProject PRJNA395315
SRA SRP113285

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101732_RNAseq_feature_count_matrix.tsv.gz 1.4 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap