|
| Status |
Public on Nov 25, 2008 |
| Title |
Role of AhR in thymocyte emigration in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Over-activation of the aryl hydrocarbon receptor by TCDD in mice leads among other phenotypes to a severe thymic atrophy accompanied by immunosuppression. TCDD causes a block in thymocyte maturation and a preferential emigration of immature CD4-CD8- DN thymocytes (recent thymic emigrants) into the periphery. As part of this study gene expression profiles from DN thymocytes and thymic emigrants were generated from TCDD and solvent control mice
Keywords: Affymetrix, TCDD, CD4-CD8- thymocytes, Thymus involution, thymic emigration, Ahr
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| |
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| Overall design |
Female, 6-8 week old C57BL6 mice were i.p. injected with 10 µg/kg TCDD. After 5 days mice were anesthezised and FITC injected into their thymi. after 24h mice were sacrificed and CD4-CD8-FITC+ cells isolated from thymus (thymocytes) and spleen (recent thymus emigrants).
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| |
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| Contributor(s) |
Temchura V, Frericks M, Esser C |
| Citation(s) |
16114106 |
| |
| Submission date |
Jan 14, 2008 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Markus Frericks |
| E-mail(s) |
fremar5@web.de
|
| Phone |
+49(0)211 3389253
|
| Fax |
+49(0)211 3190910
|
| Organization name |
Institute for Environmental Research
|
| Department |
Immunology
|
| Street address |
Auf'm Hennekamp 50
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
|
| Samples (8)
|
| GSM256739 |
CD4-CD8-_DN_thymus emigrants_corn_oil_1 |
| GSM256741 |
CD4-CD8-_DN_thymus emigrants_corn_oil_2 |
| GSM256743 |
CD4-CD8-_DN_thymus emigrants_TCDD_1 |
|
| Relations |
| BioProject |
PRJNA108365 |
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