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| Status |
Public on Jul 14, 2017 |
| Title |
Next generation sequencing of human monocyte derived dendritic cells stimulated for two hours with LPS, C5a and/or MSK inhibitor SB-747561 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to determine the full breath of C5aR and TLR crosstalk in human DC, RNA sequencing analysis on human moDC stimulated in the absence or presence of C5a and TLR4 ligand lipopolysaccharide (LPS) was performed. To minimize interference of induced positive and negative feedback loops, we analyzed gene expression after two hours of DC activation. The involvement of CREB signaling in C5aR and TLR crosstalk was investigated by including moDC stimulated in the presence of MSK inhibitor.
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| |
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| Overall design |
Human monocyte-derived dendritic cells of 4 independent donors were were sequenced on Illumina HiSeq 2000 platform
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| |
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| Contributor(s) |
Zaal A, Ten Brinke A, Moore K, Nota B |
| Citation(s) |
28733463 |
| |
| Submission date |
Jul 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Anouk Zaal |
| Organization name |
Sanquin Research
|
| Department |
Immunopathology
|
| Lab |
ten Brinke
|
| Street address |
Plesmanlaan 125
|
| City |
Amsterdam |
| State/province |
NH |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA394103 |
| SRA |
SRP111818 |
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