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| Status |
Public on Jul 13, 2017 |
| Title |
Spo11-oligo mapping of DSBs in an SK1 x S288C F1 hybrid strain |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use Spo11-oligonucleotide complexes, a byproduct of DSB formation, to map the distribution of meiotic DSBs in an SK1 x S88C F1 hybrid strain of Saccharomyces cerevisiae.
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| Overall design |
One sample, collected at 6 hr after transfer to sporulation medium
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| Web link |
https://www.biorxiv.org/content/early/2017/07/28/169870
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| Contributor(s) |
Zhu X, Keeney S |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM058673 |
Mechanism of meiotic recombination in yeast |
Sloan Kettering Institute for Cancer Research |
Scott Keeney |
| R35 GM118092 |
Mechanism and regulation of meiotic recombination |
Sloan Kettering Institute for Cancer Research |
Scott Keeney |
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| Submission date |
Jul 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Scott Keeney |
| E-mail(s) |
s-keeney@ski.mskcc.org
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| Phone |
(212) 639-5182
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| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Molecular Biology Program
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| Lab |
Keeney lab
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| Street address |
1275 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA394003 |
| SRA |
SRP111730 |
