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Series GSE101076 Query DataSets for GSE101076
Status Public on Apr 26, 2019
Title In vivo generation of post-infarct human cardiac muscle by laminin-promoted cardiovascular progenitors [LN-521 or LN-521+LN-221]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Regeneration of injured human heart muscle is limited and an unmet clinical need. There are no methods for the reproducible generation of clinical quality stem-cell-derived cardiovascular progenitors (CVPs). We identified laminin-221 (LN-221) as the most likely expressed cardiac laminin. We produced it as human recombinant protein, and showed that LN-221 promotes differentiation of pluripotent hESCs towards cardiomyocyte lineage and downregulates pluripotency and teratoma associated genes. We developed a chemically defined, xeno-free laminin-based differentiation protocol to generate CVPs. We show high reproducibility of the differentiation protocol using time-course bulk RNA sequencing developed from different hESC lines. Single-cell RNA sequencing of CVPs derived from hESC lines supported reproducibility and identified three main progenitor subpopulations. These CVPs were transplanted into myocardial infarction mice, where heart function was measured by echocardiogram and human heart muscle bundle formation was identified histologically. This method may provide clinical quality cells for use in regenerative cardiology.
Overall design Human embryonic stem cells were cultured in wells coated with LN-521 or LN-521+LN-221 using nutristem medium. RNA is extracted when cells reached confluence.
Contributor(s) Yap L, Moreno-Moral A, Petretto E, Tryggvason K
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Submission date Jul 10, 2017
Last update date May 15, 2019
Contact name Aida Moreno-Moral
Organization name Duke-NUS Medical School
Street address 8 College Road
City Singapore
ZIP/Postal code 169857
Country Singapore
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM2698972 LN521_1
GSM2698973 LN521_2
GSM2698974 LN521_3
This SubSeries is part of SuperSeries:
GSE100725 In vivo generation of post-infarct human cardiac muscle by laminin-promoted cardiovascular progenitors
BioProject PRJNA393685
SRA SRP111461

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101076_LN221_H1_samples_info.txt.gz 271 b (ftp)(http) TXT
GSE101076_LN221_TPM.txt.gz 511.2 Kb (ftp)(http) TXT
GSE101076_LN221_gene_raw_counts.txt.gz 474.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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